柏科叶绿体微卫星标记的开发
Development of chloroplast microsatellite markers for (Cupressaceae).
作者信息
Yan Ya-Dan, Li Xin-Yu, Worth James R P, Lin Xue-Ying, Ruhsam Markus, Chen Lu, Wu Xing-Tong, Wang Min-Qiu, Thomas Philip I, Wen Ya-Feng
机构信息
Central South University of Forestry and Technology Changsha Hunan 410004 People's Republic of China.
Forestry and Forest Products Research Institute Tsukuba Ibaraki 305-8687 Japan.
出版信息
Appl Plant Sci. 2019 Jul 17;7(7):e11277. doi: 10.1002/aps3.11277. eCollection 2019 Jul.
PREMISE
(Cupressaceae) is a critically endangered conifer native to China, Laos, and Vietnam, with only a few populations remaining in the wild.
METHODS AND RESULTS
Using a complete chloroplast genome sequence, we designed 70 cpSSR loci and tested them for amplification success and polymorphism in 16 samples. Ten loci were found to be polymorphic and their genetic diversity was characterized using a total of 83 individuals from three populations in China. A total of 43 haplotypes were present, the effective number of haplotypes varied from 4.55 to 13.36, and the haplotypic richness ranged from 8.04 to 16.00. Gene diversity ranged from 0.81 to 0.97 (average 0.89). The number of alleles per locus and population ranged from one to eight, and the effective number of alleles ranged from 1.00 to 3.90. All polymorphic loci were successfully amplified in the related species var. , , , and .
CONCLUSIONS
These newly developed chloroplast microsatellites will be useful for population genetic and phylogeographic analyses of and related species.
前提
（柏科）是一种极度濒危的针叶树，原产于中国、老挝和越南，野外仅存少数种群。
方法与结果
利用完整的叶绿体基因组序列，我们设计了70个叶绿体简单序列重复（cpSSR）位点，并在16个样本中测试了它们的扩增成功率和多态性。发现10个位点具有多态性，并利用来自中国三个种群的总共83个个体对其遗传多样性进行了表征。总共存在43种单倍型，单倍型的有效数量在4.55至13.36之间变化，单倍型丰富度在8.04至16.00之间。基因多样性在0.81至0.97之间（平均0.89）。每个位点和种群的等位基因数量从1到8不等，有效等位基因数量从1.00到3.90不等。所有多态性位点在相关物种变种、、和中均成功扩增。
结论
这些新开发的叶绿体微卫星将有助于对及其相关物种进行种群遗传和系统地理学分析。
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