School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, China.
Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, China.
Mol Biol Rep. 2021 Mar;48(3):3031-3036. doi: 10.1007/s11033-021-06287-z. Epub 2021 Mar 23.
Taiwania cryptomerioides Hayata is an endangered relict tree species which is endemic to mainland China, Taiwan, Myanmar, and northern Vietnam. It is an economically important tree species and has been used for reforestation in mountain areas of mainland China and Taiwan. In order to investigate its genetic diversity for conservation and restoration, we developed and characterized 15 nuclear microsatellite markers based on next-generation sequencing data. A total of 100 microsatellite primer pairs were initially designed and tested based on the restriction-site associated DNA sequencing data. 60 of 100 loci (60%) were successfully amplified, of which 42 loci exhibited polymorphism. Fifteen polymorphic microsatellite loci with clear peaks were selected for further analyses in four T. cryptomerioides populations sampled from China (Hubei, Fujian, Guizhou, and Yunnan). The number of alleles per locus ranged from 2 to 24, and the levels of observed and expected heterozygosity ranged from 0.000 to 0.950 and from 0.000 to 0.860, respectively. This set of microsatellite markers will be useful for future population genetic studies of T. cryptomerioides in East Asia.
台湾杉是中国特有的濒危孑遗树种，分布于中国大陆、台湾、缅甸和越南北部。它是一种具有经济重要性的树种，已被用于中国大陆和台湾山区的造林。为了调查其遗传多样性以进行保护和恢复，我们基于下一代测序数据开发并鉴定了 15 个核微卫星标记。最初基于限制酶相关 DNA 测序数据设计和测试了 100 个微卫星引物对。100 个位点中有 60 个（60%）成功扩增，其中 42 个位点表现出多态性。从中国（湖北、福建、贵州和云南）采集的四个台湾杉种群中选择了 15 个具有清晰峰的多态性微卫星标记进行进一步分析。每个位点的等位基因数范围为 2 到 24，观察到的和预期的杂合度水平范围分别为 0.000 到 0.950 和 0.000 到 0.860。这组微卫星标记将有助于未来东亚台湾杉的种群遗传研究。
