金顶杜鹃（杜鹃花科）的基因组和 EST 微卫星以及在其他同属物种中的交叉扩增。

Genomic and EST microsatellites for Rhododendron aureum (Ericaceae) and cross-amplification in other congeneric species.

机构信息

Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China.

出版信息

Am J Bot. 2011 Sep;98(9):e250-2. doi: 10.3732/ajb.1100153. Epub 2011 Aug 22.

DOI:10.3732/ajb.1100153

PMID:21860054

Abstract

PREMISE OF THE STUDY

Our objective of this study was to develop genomic and expressed sequence tag (EST)-derived microsatellites for the endangered species Rhododendron aureum and access their transferability in R. dauricum and R. brachycarpum. •

METHODS AND RESULTS

Twelve genomic microsatellites were isolated in R. aureum using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol and seven EST-derived microsatellites were characterized by screening the Rhododendron dbEST database of GenBank. The number of alleles per locus ranged from one to eight. The observed and expected heterozygosity varied from 0.00 to 1.00 and from 0.00 to 0.73, respectively. •

CONCLUSIONS

A total of 19 microsatellite loci were developed for R. aureum. Sixteen and 10 of these loci were successfully amplified in R. brachycarpum and R. dauricum, respectively. These microsatellite markers will have potential applications in genetic diversity and conservation genetics studies.

摘要

研究前提

本研究的目的是为濒危物种黄花杓兰开发基于基因组和表达序列标签（EST）的微卫星，并评估其在紫斑牡丹和短柱兜兰中的可转移性。

方法和结果

使用快速扩增片段长度多态性序列中重复（FIASCO）技术从黄花杓兰基因组中分离出 12 个基因组微卫星，通过筛选 GenBank 中的 Rhododendron dbEST 数据库，鉴定了 7 个 EST 衍生的微卫星。每个位点的等位基因数从 1 到 8 不等。观察到的和预期的杂合度分别从 0.00 到 1.00 和从 0.00 到 0.73 不等。