Mitchell David Alexander, Pitol Luana Oliveira, Biz Alessandra, Finkler Anelize Terezinha Jung, de Lima Luz Luiz Fernando, Krieger Nadia
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
Departamento de Engenharia Química, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
Adv Biochem Eng Biotechnol. 2019;169:27-50. doi: 10.1007/10_2019_90.
In this review, we describe our experience in building a pilot-scale packed-bed solid-state fermentation (SSF) bioreactor, with provision for intermittent mixing, and the use of this bioreactor to produce pectinases and lipases by filamentous fungi. We show that, at pilot scale, special attention must be given to several aspects that are not usually problematic when one works with laboratory-scale SSF bioreactors. For example, it can be a challenge to produce large amounts of inoculum if the fungus does not sporulate well. Likewise, at larger scales, the air preparation system needs as much attention as the bioreactor itself. Sampling can also be problematic if one wishes to avoid disrupting the bed structure. In the fermentations carried out in the pilot bioreactor, when the substrate bed contained predominantly wheat bran, the bed shrank away from the walls, providing preferential flow paths for the air and necessitating agitation of the bed. These problems were avoided by using beds with approximately 50% of sugarcane bagasse. We also show how a mathematical model that describes heat and water transfer in the bed can be a useful tool in developing appropriate control schemes. Graphical Abstract.
在本综述中，我们描述了构建中试规模的填充床固态发酵（SSF）生物反应器的经验，该反应器具备间歇混合功能，并介绍了使用此生物反应器通过丝状真菌生产果胶酶和脂肪酶的情况。我们表明，在中试规模下，必须特别关注几个方面，而这些方面在使用实验室规模的SSF生物反应器时通常不会出现问题。例如，如果真菌产孢不佳，大量接种物的制备可能会成为一项挑战。同样，在更大规模下，空气制备系统与生物反应器本身一样需要引起重视。如果希望避免破坏床层结构，取样也可能存在问题。在中试生物反应器中进行的发酵过程中，当底物床主要包含麦麸时，床层会从壁面收缩，为空气提供了优先流动路径，因此需要对床层进行搅拌。通过使用约50%甘蔗渣的床层避免了这些问题。我们还展示了一个描述床层中热量和水分传递的数学模型如何能够成为制定合适控制方案的有用工具。图形摘要。
