Food Engineering and Technology Department, Institute of Biosciences, Letters and Exact Sciences, São Paulo State University (UNESP), Cristóvão Colombo, 2265, Jardim Nazareth, São José Do Rio Preto, SP, 15054-000, Brazil.
Graduate Program of Chemical Engineering, Federal University of São Carlos (UFSCar), Rod. Washington Luiz km 235, SP-310, São Carlos, SP, 13565-905, Brazil.
Bioprocess Biosyst Eng. 2021 Mar;44(3):537-548. doi: 10.1007/s00449-020-02466-7. Epub 2020 Nov 22.
Enzymes production by solid-state cultivation in packed-bed bioreactor needs to be improved by mathematical modeling and also by experimentation. In this work, a mixture of sugarcane bagasse and wheat bran was used for the growth of the fungus Myceliophthora thermophila I-1D3b, able to secrete endoglucanase and xylanase, enzymes of interest in the second-generation ethanol production. Bench and pilot-scale bioreactors were used for the experiments, while critical parameters as bed porosity and airflow distribution were evaluated. Results showed enzymes with higher activities for the most porous medium, even though the less substrate amount to be cultivated. For the pilot-scale bioreactor, only the most porous medium was evaluated using different airflow distribution techniques. Using an inner tube for air supply resulted in more homogeneous enzyme production, with higher activities. The results here presented will be helpful for the scale-up of this class of bioreactor into industrial apparatuses.
在填充床生物反应器中进行固态培养以生产酶需要通过数学建模和实验来改进。在这项工作中，使用甘蔗渣和麦麸的混合物来培养能够分泌内切葡聚糖酶和木聚糖酶的真菌嗜热毁丝霉 I-1D3b，这些酶是第二代乙醇生产中的重要酶。使用了台架式和中试规模的生物反应器进行实验，同时评估了关键参数如床层孔隙率和气流分布。结果表明，对于最具多孔性的介质，酶具有更高的活性，尽管可用于培养的底物量较少。对于中试规模的生物反应器，仅使用不同的气流分布技术评估了最具多孔性的介质。使用内管供应空气可导致更均匀的酶生产，具有更高的活性。这里介绍的结果将有助于将这类生物反应器放大到工业设备中。
