Division of Rheumatology, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA.
Computational Biology Institute, Milken Institute School of Public Health, George Washington University, Washington, DC, USA.
Clin Exp Dermatol. 2019 Dec;44(8):882-892. doi: 10.1111/ced.13959. Epub 2019 Apr 24.
Hidradenitis suppurativa (HS) is a recurrent inflammatory disease of the apocrine sweat glands. Immune dysregulation probably contributes to the pathogenesis of HS.
To harness mRNA expression arrays to investigate the transcriptome profile in HS compared with control skin.
Illumina HumanHT-12 v4 Expression BeadChips were used to measure mRNA expression in skin samples from HS (n = 10) and abdominoplasty (n = 11) skin specimens. Differentially expressed genes were detected by fitting genewise linear models to the normalized expression data and then modelling using the web-based software Ingenuity Pathway Analysis.
The antimicrobial peptide Dermcidin and the cytokine regulator interleukin (IL)-37 were both significantly downregulated in the HS specimens (Dermcidin expression log ratio -3.93, expression P = 0.04; IL-37 expression log ratio -3.29, expression P < 0.001). Pathway analysis revealed the interferon-signalling pathway, leucocyte extravasation pathway, T helper 1 and 2 pathways and nuclear factor of activated T cells as the top-five upregulated pathways in the HS samples.
Evaluation of transcriptome patterns in HS compared with normal skin demonstrated downregulation of the antimicrobial peptide Dermcidin and the innate immune regulator IL-37, as well as upregulation of interferon pathways and pathways of leucocyte activation.
化脓性汗腺炎（HS）是一种反复发作的顶泌汗腺炎症性疾病。免疫失调可能有助于 HS 的发病机制。
利用 mRNA 表达谱来研究 HS 与对照皮肤的转录组谱。
使用 Illumina HumanHT-12 v4 Expression BeadChips 测量来自 HS（n = 10）和腹部整形术（n = 11）皮肤标本的皮肤样本中的 mRNA 表达。通过拟合基因线性模型对归一化表达数据进行拟合，然后使用基于网络的 Ingenuity Pathway Analysis 软件进行建模，来检测差异表达基因。
抗菌肽 Dermcidin 和细胞因子调节剂白细胞介素（IL）-37 在 HS 标本中均显著下调（Dermcidin 表达对数比-3.93，表达 P = 0.04；IL-37 表达对数比-3.29，表达 P < 0.001）。通路分析显示，干扰素信号通路、白细胞渗出通路、T 辅助 1 和 2 通路以及激活的 T 细胞核因子是 HS 样本中上调最多的前 5 条通路。
与正常皮肤相比，HS 转录组模式的评估显示抗菌肽 Dermcidin 和先天免疫调节剂 IL-37 的下调，以及干扰素途径和白细胞激活途径的上调。
