Fujita S, Matsubara F, Matsuda T
J Clin Microbiol. 1986 Mar;23(3):568-75. doi: 10.1128/jcm.23.3.568-575.1986.
Antibody titers against purified sulfate-soluble fraction (PSSF) obtained from cytoplasmic extracts of Candida albicans were determined retrospectively over a 2-year period for 123 cancer patients by enzyme-linked immunosorbent assay. Antibody against cell wall mannan (CWM) was also measured by the hemagglutination test and the production of precipitins by a serum interacting with a yeast cell homogenate by immunodiffusion. Invasive candidiasis determined by histological evidence at autopsy was present in 10 patients. Fourfold or greater rises in anti-CWM and anti-PSSF antibodies were detected for eight of the patients with invasive candidiasis at 14 to 22 days after the onset of fever. The immunodiffusion test was positive for four patients with invasive candidiasis. For patients with no evidence of candidiasis, significant rises in anti-CWM and anti-PSSF antibodies were observed at a frequency of 20 and 10%, respectively. The concentrations of serum mannan were sequentially measured by the enzyme-linked immunosorbent assay. Antigenemia (greater than or equal to 3 ng/ml) was found in 9 of the 10 patients with invasive candidiasis and in 2 of the 4 patients with thrush, whereas the serum of 1 of the 36 patients with no evidence of candidiasis was positive for antigen. The first antigenemia antedated significant rises in antibody levels against Candida species by 6 to 23 days.
通过酶联免疫吸附测定法，对123例癌症患者在两年期间内针对从白色念珠菌细胞质提取物中获得的纯化硫酸可溶性部分（PSSF）的抗体滴度进行了回顾性测定。还通过血凝试验测量了针对细胞壁甘露聚糖（CWM）的抗体，并通过免疫扩散法检测了血清与酵母细胞匀浆相互作用产生的沉淀素。尸检时通过组织学证据确定有10例患者发生侵袭性念珠菌病。在发热开始后第14至22天，8例侵袭性念珠菌病患者的抗CWM和抗PSSF抗体升高了四倍或更多。4例侵袭性念珠菌病患者的免疫扩散试验呈阳性。对于无念珠菌病证据的患者，抗CWM和抗PSSF抗体显著升高的频率分别为20%和10%。通过酶联免疫吸附测定法依次测量血清甘露聚糖的浓度。在10例侵袭性念珠菌病患者中有9例以及4例鹅口疮患者中有2例发现抗原血症（大于或等于3 ng/ml），而在36例无念珠菌病证据的患者中，有1例血清抗原呈阳性。首次抗原血症比针对念珠菌属的抗体水平显著升高提前6至23天出现。
