采用酶联免疫吸附测定法检测念珠菌病白血病患者的抗体和抗原血症。

Detection of antibodies and antigenemia in leukemic patients with candidiasis by enzyme-linked immunosorbent assay.

作者信息

Meckstroth K L, Reiss E, Keller J W, Kaufman L

出版信息

J Infect Dis. 1981 Jul;144(1):24-32. doi: 10.1093/infdis/144.1.24.

DOI:10.1093/infdis/144.1.24

Abstract

Standard tests and two enzyme-linked immunosorbent assays (ELISAs) were compared for detecting systemic candidiasis in 92 leukemic patients. Antibodies to Candida were detected by immunodiffusion (ID), latex agglutination (LA), and indirect ELISA; antigenemia was evaluated by indirect ELISA inhibition. In 48 patients without candidiasis (group 1), sera from 27% reacted in ID and 13% in LA tests; titers of antibody by ELISA occurred in all patients. In 34 patients without disseminated candidiasis (groups 2 and 3), Candida species were isolated; sera from 35% and 32% were positive in the ID and the LA test, respectively. In nine of 10 patients with disseminated candidiasis (group 4), LA and ID tests were negative. Seven patients in groups 1-3 without disseminated candidiasis were antigenemic (false-positive rate, 9%). Antigenemia was detected in seven of 10 patients with disseminated candidiasis; in the remaining three patients sera were drawn too early to be of diagnostic value. Thus, monitoring of immunosuppressed patients for mannan antigenemia is useful for the detection of disseminated candidiasis.

摘要

对92例白血病患者进行标准检测和两种酶联免疫吸附测定（ELISA），以检测系统性念珠菌病。通过免疫扩散（ID）、乳胶凝集试验（LA）和间接ELISA检测抗念珠菌抗体；通过间接ELISA抑制法评估菌血症。在48例无念珠菌病的患者（第1组）中，27%的血清在ID试验中呈反应性，13%在LA试验中呈反应性；所有患者通过ELISA检测到抗体滴度。在34例无播散性念珠菌病的患者（第2组和第3组）中，分离出念珠菌属；ID试验和LA试验中分别有35%和32%的血清呈阳性。在10例播散性念珠菌病患者（第4组）中有9例，LA和ID试验均为阴性。第1 - 3组中7例无播散性念珠菌病的患者存在菌血症（假阳性率为9%）。10例播散性念珠菌病患者中有7例检测到菌血症；其余3例患者采血时间过早，无诊断价值。因此，监测免疫抑制患者的甘露聚糖菌血症对检测播散性念珠菌病很有用。