Langer T, Kaneko C R, Scudder C A, Fuchs A F
J Comp Neurol. 1986 Mar 15;245(3):379-400. doi: 10.1002/cne.902450307.
The abducens nucleus is a central coordinating element in the generation of conjugate horizontal eye movements. As such, it should receive and combine information relevant to visual fixation, saccadic eye movements, and smooth eye movements evoked by vestibular and visual stimuli. To reveal possible sources of these signals, we retrogradely labeled the afferents to the abducens nucleus by electrophoretically injecting horseradish peroxidase into an abducens nucleus in four monkeys and two cats. The histologic material was processed by the tetramethyl benzidine (TMB) method of Mesulam. In both species the largest source of afferents to the abducens nucleus was bilateral projections from the ventrolateral vestibular nucleus and the rostral pole of the medial vestibular nucleus. Scattered neurons were also labeled in the middle and caudal levels of the medial vestibular nucleus. Large numbers of neurons were labeled in the ventral margin of the nucleus prepositus hypoglossi in the cat and in the common margin of the nucleus prepositus and the medial vestibular nucleus in the monkey, a region we call the marginal zone. Substantial numbers of retrogradely labeled neurons were found in the dorsomedial pontine reticular formation both caudal and rostral to the abducens nuclei. In the monkey, large numbers of labeled neurons were present in the contralateral medial rectus subdivision of the oculomotor complex, while smaller numbers occurred in the ipsilateral medial rectus subdivision and elsewhere in the oculomotor complex. In the cat, large numbers of retrogradely labeled cells were present in a small periaqueductal gray nucleus immediately dorsal to the caudal pole of the oculomotor complex, and a few labeled neurons were also dispersed through the caudal part of the oculomotor complex. Occasional labeled neurons were present in the contralateral superior colliculus in both species. The size and distribution of the labeled neurons within the intermediate gray differed dramatically in the two species. In the cat, the retrogradely labeled neurons were very large and occurred predominantly in the central region of the colliculus, while in the monkey, they were small to intermediate in size and were distributed more uniformly within the middle gray. Among the afferent populations present in the monkey, but not in the cat, was a group of scattered neurons in the ipsilateral rostral interstitial nucleus of the medial longitudinal fasciculus and a denser, bilateral population in the interstitial nucleus of Cajal.(ABSTRACT TRUNCATED AT 400 WORDS)
展神经核是共轭水平眼球运动产生过程中的一个中枢协调元件。因此，它应该接收并整合与视觉注视、眼球跳动以及前庭和视觉刺激诱发的平稳眼球运动相关的信息。为了揭示这些信号的可能来源，我们通过将辣根过氧化物酶电泳注入4只猴子和2只猫的展神经核，逆行标记了展神经核的传入纤维。组织学材料采用Mesulam的四甲基联苯胺（TMB）方法进行处理。在这两个物种中，展神经核传入纤维的最大来源是来自腹外侧前庭核和内侧前庭核头端的双侧投射。在内侧前庭核的中、尾段也有散在的神经元被标记。在猫的舌下前置核腹侧边缘以及猴子的舌下前置核与内侧前庭核的共同边缘（我们称之为边缘区）有大量神经元被标记。在展神经核尾侧和头侧的脑桥背内侧网状结构中发现了大量逆行标记的神经元。在猴子中，动眼神经复合体对侧的内直肌亚核中有大量标记神经元，而同侧内直肌亚核和动眼神经复合体其他部位的标记神经元较少。在猫中，大量逆行标记细胞出现在紧邻动眼神经复合体尾端背侧的一个小的导水管周围灰质核中，并且有一些标记神经元也散布在动眼神经复合体的尾侧部分。在这两个物种的对侧上丘中偶尔也有标记神经元。两个物种中间灰质内标记神经元的大小和分布差异很大。在猫中，逆行标记神经元非常大，主要出现在丘的中央区域，而在猴子中，它们大小从小到中等，并且在中间灰质内分布更均匀。在猴子中存在但在猫中不存在的传入神经元群体包括同侧内侧纵束间质性核中的一组散在神经元以及 Cajal 间质核中更密集的双侧神经元群体。（摘要截于400字）
