Gonzalo-Ruiz A, Leichnetz G R, Smith D J
Department of Anatomy, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
J Comp Neurol. 1988 Feb 22;268(4):508-26. doi: 10.1002/cne.902680404.
Cerebellar projections to oculomotor-related brainstem regions were studied in four groups of New World (capuchin, squirrel) monkeys by using the retrograde transport of horseradish peroxidase (HRP) to determine the origin of the principal cerebellar influence on eye movement. Group A monkeys had HRP injections or transcannular HRP gel implants into the oculomotor complex (OMC), the largest of which involved adjacent paraoculomotor nuclei (e.g., ventral periaqueductal gray, PAG; nucleus of Darkschewitsch, ND; medial accessory nucleus of Bechterew, MAB; dorsomedial parvicellular red nucleus, dmPRN). All of these cases contained large numbers of retrogradely labeled cells in cell group Y. Whereas the smallest OMC injection only labeled a few cells in the dentate nucleus (DN), injections involving paraoculomotor nuclei produced labeling in all of the cerebellar nuclei except the basal interstitial nucleus (BIN). Injections extending into the ND and MAB produced particularly heavy labeling within the interposed nuclei. Group B monkeys had injections/implants into the medial pontine tegmentum and dorsomedial basilar pons. The pontine tegmental cases contained labeled cells in all cerebellar nuclei, but the DN was the most heavily labeled when the implant involved the nucleus reticularis tegmenti pontis (NRTP). Cases with injections into the caudal medial pontine tegmentum (nucleus reticularis pontis caudalis, NRPC), including the physiological paramedian pontine reticular formation (PPRF), but not NRTP, contained the largest number of labeled cells in the fastigial nucleus (FN) and lacked retrograde labeling in the DN. Dorsomedial basilar pontine cases contained almost no labeled cells in the FN, anterior interpositus nucleus (AIN), and posterior interpositus nucleus (PIN) but did contain DN labeling when the injection involved the NRTP. Two dorsomedial pontine tegmental cases and one dorsomedial basilar pontine case had more labeled cells in the BIN than in other cases. Tegmental cases also contained a few labeled cells in cell group Y. Group C monkeys had injections into the parvicellular red nucleus (PRN) and had their heaviest labeling in the DN, although the AIN and PIN also contained labeled cells. The FN, BIN, and cell group Y, on the other hand, contained almost no labeling. Group D consisted of monkeys which had injections into the intermediate and deep superior colliculus (SC). These cases contained the largest numbers of labeled cells in the PIN and a lesser number in the ventrolateral FN. The DN, AIN, BIN, and cell group Y lacked labeled neurons in these cases.(ABSTRACT TRUNCATED AT 400 WORDS)
通过辣根过氧化物酶（HRP）逆行运输法，在四组新大陆（卷尾猴、松鼠猴）猴子中研究了小脑向与动眼相关的脑干区域的投射，以确定小脑对眼球运动主要影响的起源。A组猴子将HRP注射或经套管的HRP凝胶植入动眼神经复合体（OMC），其中最大的注射涉及相邻的动眼旁核（如腹侧导水管周围灰质，PAG；达克谢维奇核，ND；别赫捷列夫内侧副核，MAB；背内侧小细胞红核，dmPRN）。所有这些病例在Y细胞群中都含有大量逆行标记的细胞。虽然最小的OMC注射仅在齿状核（DN）中标记了少数细胞，但涉及动眼旁核的注射在除基底间质核（BIN）外的所有小脑核中都产生了标记。延伸至ND和MAB的注射在间位核中产生了特别强烈的标记。B组猴子将注射物/植入物注入脑桥内侧被盖和背内侧基底脑桥。脑桥被盖病例在所有小脑核中都含有标记细胞，但当植入物涉及脑桥被盖网状核（NRTP）时，DN的标记最为强烈。注射到尾侧脑桥内侧被盖（脑桥尾侧网状核，NRPC），包括生理性旁正中脑桥网状结构（PPRF）但不包括NRTP的病例，在顶核（FN）中含有最多数量的标记细胞，而在DN中缺乏逆行标记。背内侧基底脑桥病例在FN、前间位核（AIN）和后间位核（PIN）中几乎没有标记细胞，但当注射涉及NRTP时，确实含有DN标记。两个脑桥内侧被盖病例和一个背内侧基底脑桥病例在BIN中的标记细胞比其他病例更多。被盖病例在Y细胞群中也含有一些标记细胞。C组猴子将注射物注入小细胞红核（PRN），其在DN中的标记最重，尽管AIN和PIN中也含有标记细胞。另一方面，FN、BIN和Y细胞群几乎没有标记。D组由将注射物注入中脑和深层上丘（SC）的猴子组成。这些病例在PIN中含有最多数量的标记细胞，在腹外侧FN中含有较少数量的标记细胞。在这些病例中，DN、AIN、BIN和Y细胞群缺乏标记神经元。（摘要截于400字）
