Balaban E P, Simon T R, Sheehan R G, Frenkel E P
J Lab Clin Med. 1986 Apr;107(4):306-14.
The in vitro use of the radioisotope indium 111 (111In) was examined as a radiolabel for lymphocytes obtained from both normal individuals and patients with a variety of lymphoid malignancies. Successful cell labeling requires a chelator. The traditional agent oxine, has proved to be toxic to the lymphoid lineage. Cellular uptake of 111In mediated by the chelator oxine was compared with that of a new chelator, tropolone. Oxine provided better labeling efficiency (48%) than tropolone (35%) for the labeling of normal lymphocytes. By contrast, lymphocytes from patients with chronic lymphocytic leukemia had a nearly twofold greater labeling efficiency when tropolone was substituted for oxine. Further studies demonstrated that tropolone induced functional injury to lymphocytes when mitogenic response to concanavalin A, pokeweed mitogen, and phytohemagglutinin was assessed. Similar toxicity was found when tropolone was compared with oxine. In addition, tropolone produced damaging structural changes seen by both scanning and transmission electron microscopic examination. These changes were both variable and not predictable. Shortening of the incubation time of the chelator with the cell provided the least amount of cellular injury. These findings suggest that tropolone be used as an alternative mediator of lymphocyte labeling with 111In only under critically defined conditions.
对放射性同位素铟111（¹¹¹In）在体外作为从正常个体和患有各种淋巴恶性肿瘤的患者中获取的淋巴细胞的放射性标记物的用途进行了研究。成功的细胞标记需要一种螯合剂。传统试剂8-羟基喹啉已被证明对淋巴谱系有毒性。将由螯合剂8-羟基喹啉介导的¹¹¹In的细胞摄取与一种新的螯合剂托酚酮的细胞摄取进行了比较。对于正常淋巴细胞的标记，8-羟基喹啉提供了比托酚酮更好的标记效率（48%），而托酚酮的标记效率为35%。相比之下，当用托酚酮替代8-羟基喹啉时，慢性淋巴细胞白血病患者的淋巴细胞标记效率几乎提高了两倍。进一步的研究表明，当评估对刀豆球蛋白A、商陆有丝分裂原和植物血凝素的促有丝分裂反应时，托酚酮会诱导淋巴细胞发生功能性损伤。当将托酚酮与8-羟基喹啉进行比较时，发现了类似的毒性。此外，通过扫描电子显微镜和透射电子显微镜检查发现，托酚酮会产生破坏性的结构变化。这些变化既多变又不可预测。缩短螯合剂与细胞的孵育时间造成的细胞损伤最少。这些发现表明，只有在严格限定的条件下，托酚酮才可作为¹¹¹In标记淋巴细胞的替代介质。
