Vallabhajosula S, Machac J, Goldsmith S J, Lipszyc H, Badimon L, Rand J, Fuster V
J Nucl Med. 1986 Nov;27(11):1669-74.
The effect of labeling media on the kinetics of[111In]platelets was evaluated by performing a paired crossover study in eight normal human subjects using tropolone and oxine methods. Platelets were labeled in autologous plasma with [111In]tropolone (In-tr) and in ACD-saline with [111In]oxine (In-ox) and reinjected. Starting at 1 hr, ten blood samples were obtained over an 8-day period. The in vivo platelet recovery was higher at 1 hr and throughout the 8 days of study with In-tr and the gamma camera images showed less uptake in liver and spleen than with In-ox. When platelet life-span (PLS) was estimated using all ten samples, only linear regression showed that the platelet life-span was longer with In-tr (10.7 +/- 1.5) than with In-ox (9.5 +/- 0.8). When the PLS was estimated excluding the 1-hr sample point, the life-span of platelets was significantly longer with In-tr than with In-ox based on three out of four models of curve fitting. These results demonstrate that platelets labeled with In-tr in plasma are preserved better in circulation and have equal or longer life-span than platelets labeled with In-ox in ACD-saline.
通过在8名正常人类受试者中使用托酚酮和8-羟基喹啉方法进行配对交叉研究，评估标记介质对[111In]血小板动力学的影响。血小板在自体血浆中用[111In]托酚酮（In-tr）标记，在ACD-盐水中用[111In]8-羟基喹啉（In-ox）标记后重新注入。从1小时开始，在8天内采集10份血样。与In-ox相比，In-tr在1小时时以及在整个8天的研究中体内血小板回收率更高，γ相机图像显示肝脏和脾脏的摄取更少。当使用所有10份样本估计血小板寿命（PLS）时，只有线性回归显示In-tr（10.7±1.5）标记的血小板寿命比In-ox（9.5±0.8）标记的更长。当排除1小时样本点估计PLS时，基于四种曲线拟合模型中的三种，In-tr标记的血小板寿命明显长于In-ox标记的血小板寿命。这些结果表明，血浆中用In-tr标记的血小板在循环中保存得更好，并且与ACD-盐水中用In-ox标记的血小板具有相同或更长的寿命。
