保持植物组织形态，用于可视化天门冬中成像质谱断层扫描的分割和相关分析中的代谢物特征。
Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis.
机构信息
RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan.
Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8675, Japan.
出版信息
Metabolomics. 2019 Feb 14;15(2):24. doi: 10.1007/s11306-019-1486-5.
INTRODUCTION
Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is a powerful approach for visualizing the localization of metabolites.
OBJECTIVES
A method to keep the shape of plant tissue needs to be developed for MALDI-IMS.
METHODS
The method was developed using transfer tape and double-sided conductive tape.
RESULTS
MALDI-IMS analysis using the developed method enabled to perform segmentation and correlation analysis of mass features.
CONCLUSION
This proof-of-concept study showed that rutin localizes in the epidermis, developing tissue, and protoxylem in Asparagus officinalis.
简介
基质辅助激光解吸/电离成像质谱（MALDI-IMS）是一种用于可视化代谢物定位的强大方法。
目的
需要开发一种保持植物组织形状的 MALDI-IMS 方法。
方法
该方法使用转移带和双面导电带开发。
结果
使用开发的方法进行 MALDI-IMS 分析，能够对质量特征进行分割和相关分析。
结论
这项概念验证研究表明，芦丁在天门冬科植物天门冬中定位于表皮、发育组织和原木质部。
Zotero 插件