暴露于碳纳米管的大肠杆菌噬菌体休克蛋白和基因反应。

Phage shock protein and gene responses of Escherichia coli exposed to carbon nanotubes.

机构信息

Environmental and Conservation Sciences Program, North Dakota State University, Fargo, ND 58108, USA; Biology Department, Dalat University, Dalat, Lamdong, Viet Nam.

Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Environmental Science Research Center (ESRC), Chiang Mai University, Chiang Mai, 50200, Thailand.

出版信息

Chemosphere. 2019 Jun;224:461-469. doi: 10.1016/j.chemosphere.2019.02.159. Epub 2019 Feb 25.

DOI:10.1016/j.chemosphere.2019.02.159

PMID:30831497

Abstract

Two-dimensional electrophoretic, western blotting, and quantitative polymerase chain reaction analyses of Escherichia coli cells exposed to pristine single walled carbon nanotubes (SWCNTs), and hydroxyl and carboxylic functionalized SWCNTs (SWCNT-OHs and SWCNT-COOHs) were conducted. SWCNT concentration and length were experimental variables. Exposing E. coli cells to SWCNTs led to changes in protein and gene expressions. Several proteins altered their regulations at a low SWCNT concentration (10 μg/ml) and were shut down at a high SWCNT concentration (100 μg/ml). The expressions of the phage shock protein (psp) operon including pspA, pspB, and pspC genes responded to the membrane stressors, SWCNTs, were also examined. While pspA and pspC expressions were influenced by the length, concentration, and functional groups of SWCNTs, pspB expression was not induced by SWCNTs. The alterations in phage shock protein and gene expressions indicated that SWCNTs caused cell membrane perturbation.

摘要

对暴露于原始单壁碳纳米管 (SWCNT) 以及羟基和羧基功能化 SWCNT (SWCNT-OH 和 SWCNT-COOH) 的大肠杆菌细胞进行二维电泳、western 印迹和定量聚合酶链式反应分析。SWCNT 浓度和长度是实验变量。暴露于 SWCNT 会导致大肠杆菌细胞中的蛋白质和基因表达发生变化。一些蛋白质在低 SWCNT 浓度（10μg/ml）下改变其调节方式，而在高 SWCNT 浓度（100μg/ml）下则被关闭。噬菌体休克蛋白（psp）操纵子的表达，包括 pspA、pspB 和 pspC 基因，也受到膜应激物 SWCNT 的影响。虽然 pspA 和 pspC 的表达受到 SWCNT 的长度、浓度和官能团的影响，但 pspB 的表达不受 SWCNT 的诱导。噬菌体休克蛋白和基因表达的改变表明，SWCNT 导致了细胞膜扰动。