Department of Biology, Missouri State University, 901 S National, Springfield, MO 65897, USA.
Jordan Valley Innovation Center, Missouri State University, 542 N Boonville, Springfield, MO 65806, USA.
Genes (Basel). 2021 May 23;12(6):794. doi: 10.3390/genes12060794.
Next-generation sequencing (NGS) technology has revolutionized sequence-based research. In recent years, high-throughput sequencing has become the method of choice in studying the toxicity of chemical agents through observing and measuring changes in transcript levels. Engineered nanomaterial (ENM)-toxicity has become a major field of research and has adopted microarray and newer RNA-Seq methods. Recently, nanotechnology has become a promising tool in the diagnosis and treatment of several diseases in humans. However, due to their high stability, they are likely capable of remaining in the body and environment for long periods of time. Their mechanisms of toxicity and long-lasting effects on our health is still poorly understood. This review explores the effects of three ENMs including carbon nanotubes (CNTs), quantum dots (QDs), and Ag nanoparticles (AgNPs) by cross examining publications on transcriptomic changes induced by these nanomaterials.
下一代测序（NGS）技术彻底改变了基于序列的研究。近年来，高通量测序已成为通过观察和测量转录水平变化来研究化学试剂毒性的首选方法。 工程纳米材料（ENM）毒性已成为一个主要的研究领域，并采用了微阵列和更新的 RNA-Seq 方法。 最近，纳米技术已成为人类几种疾病诊断和治疗的有前途的工具。 然而，由于它们的高稳定性，它们很可能能够在体内和环境中长时间存在。 它们的毒性机制及其对我们健康的长期影响仍知之甚少。 本综述通过交叉检查这些纳米材料引起的转录组变化的出版物，探讨了三种 ENM（包括碳纳米管（CNTs）、量子点（QDs）和 Ag 纳米颗粒（AgNPs））的作用。
