一种使用金属窗的高压池，通过小角中子散射研究高达600兆帕压力下分子溶液的结构。

A high pressure cell using metallic windows to investigate the structure of molecular solutions up to 600 MPa by small-angle neutron scattering.

作者信息

Annighöfer Burkhard, Hélary Arnaud, Brûlet Annie, Colas de la Noue Alexandre, Loupiac Camille, Combet Sophie

机构信息

Laboratoire Léon-Brillouin (LLB), UMR 12 CEA-CNRS, Université Paris-Saclay, CEA-Saclay, F-91191 Gif-sur-Yvette CEDEX, France.

出版信息

Rev Sci Instrum. 2019 Feb;90(2):025106. doi: 10.1063/1.5051765.

DOI:10.1063/1.5051765

PMID:30831774

Abstract

We report on a high pressure (HP) cell designed for the determination of the structure of molecular solutions by small-angle neutron scattering (SANS). The HP cell is fitted up with two thick metallic windows that make the device very resistant under hydrostatic pressures up to 600 MPa (or 6 kbar). The metallic windows are removable, offering the possibility to adapt the HP cell to a given study with the pressure desired on an appropriate spatial range to study the structure of various molecular solutions by SANS. In this context, we report the absorption, transmission, and scattering properties of different metallic windows. Finally, we describe, as a proof of principle, the solution structure changes of myoglobin, a small globular protein.

摘要

我们报道了一种高压（HP）池，其设计用于通过小角中子散射（SANS）测定分子溶液的结构。该高压池配备有两个厚金属窗，这使得该装置在高达600兆帕（或6千巴）的静水压力下具有很高的耐受性。金属窗是可拆卸的，这使得高压池有可能根据特定研究的需要，在合适的空间范围内施加所需压力，以通过SANS研究各种分子溶液的结构。在此背景下，我们报道了不同金属窗的吸收、透射和散射特性。最后，作为原理验证，我们描述了一种小球形蛋白质——肌红蛋白的溶液结构变化。