Mandal B, Pappu H R, Culbreath A K
Department of Plant Pathology, University of Georgia, Coastal Plain Experiment Station, Tifton 31793.
Plant Dis. 2001 Dec;85(12):1259-1263. doi: 10.1094/PDIS.2001.85.12.1259.
Evaluation of peanut germ plasm for Tomato spotted wilt virus (TSWV) resistance has been slowed by the difficulty in achieving a high rate of mechanical transmission of the virus to peanut. In this study, improvements were made and a highly efficient mechanical transmission protocol was developed. Several factors that affect the transmission efficiency were identified. Use of two antioxidants (sodium sulfite and mercaptoethanol) and two abrasives (Celite and Carborundum) and application of the inoculum by rubbing with a cotton swab dipped in the inoculum as well as pricking with an inoculation needle resulted in a significantly higher transmission rate. The most susceptible growth stage of peanut to TSWV inoculation was 2 to 3 days after germination (6 to 7 days after planting). The inoculation protocol consistently resulted in a higher percentage of infected plants from different sources of inoculum such as infected peanut, tobacco, and tomato.
由于难以实现将番茄斑萎病毒（TSWV）高效机械传播到花生上，花生种质对TSWV抗性的评估工作进展缓慢。在本研究中，对方法进行了改进并开发出了一种高效的机械传播方案。确定了几个影响传播效率的因素。使用两种抗氧化剂（亚硫酸钠和巯基乙醇）和两种研磨剂（硅藻土和金刚砂），并用蘸有接种物的棉签擦拭以及用接种针穿刺的方式接种接种物，可使传播率显著提高。花生对TSWV接种最敏感的生长阶段是发芽后2至3天（种植后6至7天）。该接种方案始终能使来自不同接种源（如受感染的花生、烟草和番茄）的植物感染率更高。
