Thompson J R, Jelkmann W
BBA, Institut für Pflanzenschutz im Obstbau, Schwabenheimer Straße 101, D- 69221, Dossenheim, Germany.
Plant Dis. 2003 Apr;87(4):385-390. doi: 10.1094/PDIS.2003.87.4.385.
Strawberry mottle virus (SMoV) is probably the most important virus to infect strawberry (Fragaria spp.). All species of strawberry are susceptible to SMoV, resulting in severe losses both in fruit and runner yield. However, due to the absence of definitive symptoms in commercial varieties, the only effective means of detecting SMoV is by transmission to susceptible indicator plants. In this study, we describe a reverse transcription-polymerase chain reaction (RT-PCR) method for the detection of SMoV in Fragaria spp. with the use of primers specific for the noncoding regions (NCR) of both RNA1 and RNA2 of the virus. Using this method, all of 16 isolates from various geographical origins were positive for SMoV. Partial sequences of a 327-nt long coding region were obtained for the putative large coat protein of all isolates by RT-PCR using degenerate primers. Nucleotide identities between isolates ranged from 72.8 to 99.7%. A 546-nt sequence of the putative polymerase gene of nine isolates was obtained by RT-PCR and compared. Nucleotide identities ranged from 73.4 to 100%. There was a clear tendency for isolates to group according to their geographical origin. Sequence data obtained of the NCR show four completely conserved regions of 20 or more bases.
草莓斑驳病毒（SMoV）可能是侵染草莓（草莓属）的最重要病毒。所有草莓品种都易感染SMoV，导致果实和匍匐茎产量严重损失。然而，由于商业品种中缺乏明确的症状，检测SMoV的唯一有效方法是将其接种到敏感指示植物上。在本研究中，我们描述了一种逆转录-聚合酶链反应（RT-PCR）方法，用于利用针对该病毒RNA1和RNA2非编码区（NCR）的特异性引物检测草莓属植物中的SMoV。使用该方法，来自不同地理来源的16个分离株均检测出SMoV呈阳性。通过使用简并引物进行RT-PCR，获得了所有分离株假定的大外壳蛋白327个核苷酸长编码区的部分序列。分离株之间的核苷酸同一性范围为72.8%至99.7%。通过RT-PCR获得了9个分离株假定的聚合酶基因546个核苷酸的序列并进行了比较。核苷酸同一性范围为73.4%至100%。分离株明显有根据其地理来源进行分组的趋势。NCR的序列数据显示有4个20个或更多碱基的完全保守区域。
