Xu Tengfei, Gao Dehang, Wu Mengmeng, Wang Hongqing, He Chengyong
Institute of Horticulture, Sichuan Academy of Agricultural Sciences, Chengdu 610000, China.
Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement in Southwest, Ministry of Agriculture and Rural Affairs, Chengdu 610000, China.
Plants (Basel). 2025 Jul 27;14(15):2330. doi: 10.3390/plants14152330.
SMoV and SVBV are two major viruses that pose significant threats to the global strawberry industry. Both are latent viruses, making early detection difficult due to their uneven distribution and low concentration in host tissues. Traditional RT-PCR techniques are insufficient for precise and quantitative detection. In this study, TaqMan RT-qPCR detection systems for SMoV and SVBV were established for application in practical production settings, enabling accurate, rapid, and efficient detection of strawberry viruses. When viral accumulation in plants is low, the highly sensitive TaqMan RT-qPCR technique allows for accurate quantification, facilitating the early identification of infected plants and preventing large-scale outbreaks in cultivation areas. The development of a duplex TaqMan RT-qPCR assay enables simultaneous quantification of SMoV and SVBV in a single reaction, improving detection efficiency and providing technical support for risk assessment and effective control of strawberry viral diseases.
草莓轻型黄边病毒（SMoV）和草莓镶脉病毒（SVBV）是对全球草莓产业构成重大威胁的两种主要病毒。二者均为潜伏病毒，因其在寄主组织中分布不均且浓度较低，早期检测困难。传统的逆转录聚合酶链反应（RT-PCR）技术不足以进行精确和定量检测。本研究建立了用于SMoV和SVBV的TaqMan逆转录定量聚合酶链反应（RT-qPCR）检测系统，以应用于实际生产环境，实现对草莓病毒的准确、快速和高效检测。当植物中的病毒积累量较低时，高灵敏度的TaqMan RT-qPCR技术可实现准确定量，有助于早期识别受感染植株，防止种植区大规模爆发。双重TaqMan RT-qPCR检测方法的开发能够在单一反应中同时定量SMoV和SVBV，提高检测效率，为草莓病毒病的风险评估和有效防控提供技术支持。
