Bhat R G, Subbarao K V
Department of Plant Pathology, University of California, Davis, c/o U.S. Agricultural Research Station, 1636 E. Alisal St., Salinas, CA 93905.
Plant Dis. 2001 Feb;85(2):141-146. doi: 10.1094/PDIS.2001.85.2.141.
Isolates of Verticillium dahliae from 15 different hosts and V. albo-atrum from alfalfa were tested for their ability to cause wilt on broccoli using a root-dip inoculation method. None of the isolates caused vascular discoloration in broccoli except those from cabbage and cauliflower that were weakly pathogenic. Broccoli cultivars Baccus, Greenbelt, Parasol, Patriot, and Symphony showed resistance to Verticillium infection. Re-isolated strains from fresh samples of internally discolored broccoli and cauliflower root tissues were unable to cause disease symptoms on re-inoculation of broccoli, but caused severe disease on cauliflower. Inoculation of 5-, 7-, 9-, or 11-week-old plants did not alter the resistance in broccoli or the susceptibility in cauliflower against Verticillium. Immunity of broccoli against infection by isolates of V. dahliae from non-crucifer hosts, its resistance against crucifer isolates, and as previously described, attrition of V. dahliae microsclerotia in soil by broccoli residue, coupled with its importance as a commercial vegetable, make broccoli an attractive rotation crop for the management of Verticillium wilt in many cropping systems.
采用浸根接种法，对从15种不同寄主分离得到的大丽轮枝菌菌株以及从苜蓿分离得到的黑白轮枝菌菌株进行了测试，以检测它们引发西兰花枯萎病的能力。除了来自甘蓝和花椰菜的弱致病菌株外，其他菌株均未在西兰花中引起维管束变色。西兰花品种巴克斯、绿地、阳伞、爱国者和交响乐表现出对轮枝菌感染的抗性。从内部变色的西兰花和花椰菜根组织新鲜样本中重新分离出的菌株，再次接种到西兰花上时无法引发病害症状，但接种到花椰菜上会引发严重病害。对接种5周、7周、9周或11周龄的植株，并未改变西兰花的抗性或花椰菜对轮枝菌的易感性。西兰花对非十字花科寄主的大丽轮枝菌菌株感染具有免疫性，对十字花科菌株具有抗性，且如前所述，西兰花残茬可使土壤中的大丽轮枝菌微菌核损耗，再加上其作为商业蔬菜的重要性，使得西兰花成为许多种植系统中防治黄萎病的一种有吸引力的轮作作物。
