Font I, Martínez-Culebras P, Jordá C
Departament Vegetal Production, Plant Pathology (Agrónomos), Universidad Politécnica, Cno. De Vera 14, Valencia, Spain.
Plant Dis. 2000 Sep;84(9):1046. doi: 10.1094/PDIS.2000.84.9.1046C.
In autumn of 1999 and winter-spring 2000, tomato (Lycopersicon) crops grown in the Regions of Las Palmas de Gran Canaria and Tenerife (Canary Islands) showed upward curling of leaves, yellowing of leaf margins, crumpling of new leaves, reduction of leaflet area, and stunting of shoots. These symptoms were similar to those described for tomato yellow leaf curl disease. Symptomatic samples were collected from Las Palmas de Gran Canaria (33 samples) and Tenerife (45 samples) for polymerase chain reaction (PCR) identification analysis. The degenerate primers pair of Begomovirus (AV494/AC1048) (3) was used to amplify the "core" region of the capsid protein gene. Two tomato plants experimentally infected with Tomato yellow leaf curl virus-Is (TYLCVIs) or TYLCV-Sar served as positive controls. Electrophoretic analysis of all samples showed a single fragment of the expected size (550 bp). To identify the type of TYLCV (TYLCV-Sar or TYLCV-Is), the PCR products were digested by endonucleases (AluI, HaeIII, HpaII, RsaI, Sau3A, TaqI, DdeI, and ScrFI). Twenty-six samples from Las Palmas de Gran Canaria showed the same restriction pattern of TYLCV-Sar, and seven samples from Las Palmas de Gran Canaria and all 45 samples from Tenerife showed the same restriction pattern of TYLCV-Is. These results confirm that TYLCV-Sar and TYLCV-Is are present in Las Palmas de Gran Canaria and TYLCV-Is is present in Tenerife. The presence of TYLCV-Is in Morocco (2) and TYLCV-Sar in the Canary Islands and Morocco has been recently described (1). However, this is the first report of TYLCV-Is in the Canary Islands. References: (1) F. Monci et al. Plant Dis. 84:490, 2000. (2) M. Peterschmitt et al. Plant Dis. 83:1074, 1999. (3) S. D. Wyatt et al. Phytopathology 86:1288, 1996.
1999年秋季以及2000年冬春季节，大加那利岛拉斯帕尔马斯和特内里费岛（加那利群岛）种植的番茄作物出现叶片向上卷曲、叶缘发黄、新叶皱缩、小叶面积减小以及嫩枝发育不良的症状。这些症状与番茄黄化曲叶病所描述的症状相似。从大加那利岛拉斯帕尔马斯（33个样本）和特内里费岛（45个样本）采集了有症状的样本，用于聚合酶链反应（PCR）鉴定分析。使用双生病毒的简并引物对（AV494/AC1048）（3）扩增衣壳蛋白基因的“核心”区域。用番茄黄化曲叶病毒-Is（TYLCV-Is）或番茄黄化曲叶病毒-Sar（TYLCV-Sar）实验性感染的两株番茄植株作为阳性对照。对所有样本的电泳分析显示出预期大小（550 bp）的单一片段。为了鉴定番茄黄化曲叶病毒（TYLCV）的类型（TYLCV-Sar或TYLCV-Is），PCR产物用核酸内切酶（AluI、HaeIII、HpaII、RsaI、Sau3A、TaqI、DdeI和ScrFI）进行消化。大加那利岛拉斯帕尔马斯的26个样本显示出与TYLCV-Sar相同的限制性酶切图谱，大加那利岛拉斯帕尔马斯的7个样本以及特内里费岛的所有45个样本显示出与TYLCV-Is相同的限制性酶切图谱。这些结果证实大加那利岛拉斯帕尔马斯存在TYLCV-Sar和TYLCV-Is，特内里费岛存在TYLCV-Is。最近已有摩洛哥存在TYLCV-Is（2）以及加那利群岛和摩洛哥存在TYLCV-Sar的报道（1）。然而，这是加那利群岛存在TYLCV-Is的首次报道。参考文献：（1）F. Monci等人，《植物病害》84:490，2000年。（2）M. Peterschmitt等人，《植物病害》83:1074，1999年。（3）S. D. Wyatt等人，《植物病理学》86:1288，1996年。
