Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio al. 7, LT-10257 Vilnius, Lithuania.
Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio al. 7, LT-10257 Vilnius, Lithuania.
Viruses. 2019 Mar 1;11(3):208. doi: 10.3390/v11030208.
Nucleotides, peptides and proteins serve as a scaffold material for self-assembling nanostructures. In this study, the production of siphovirus vB_EcoS_NBD2 (NBD2) recombinant tail tube protein gp39 reached approximately 33% and 27% of the total cell protein level in and expression systems, respectively. A simple purification protocol allowed us to produce a recombinant gp39 protein with 85%⁻90% purity. The yield of gp39 was 2.9 ± 0.36 mg/g of wet cells and 0.85 ± 0.33 mg/g for cells. The recombinant gp39 self-assembled into well-ordered tubular structures (polytubes) in vivo in the absence of other phage proteins. The diameter of these structures was the same as the diameter of the tail of phage NBD2 (~12 nm). The length of these structures varied from 0.1 µm to >3.95 µm, which is 23-fold the normal NBD2 tail length. Stability analysis demonstrated that the polytubes could withstand various chemical and physical conditions. These polytubes show the potential to be used as a nanomaterial in various fields of science.
核苷酸、肽和蛋白质可作为自组装纳米结构的支架材料。在这项研究中，噬菌粒 vB_EcoS_NBD2（NBD2）重组尾部管蛋白 gp39 的产量在 和 表达系统中分别达到了总细胞蛋白水平的约 33%和 27%。一个简单的纯化方案允许我们生产出纯度为 85%-90%的重组 gp39 蛋白。从湿 细胞中，gp39 的产量为 2.9 ± 0.36 mg/g，从 细胞中，gp39 的产量为 0.85 ± 0.33 mg/g。重组的 gp39 在没有其他噬菌体蛋白的情况下在体内自组装成有序的管状结构（多管）。这些结构的直径与噬菌体 NBD2 的尾部直径相同（~12nm）。这些结构的长度从 0.1µm 到>3.95µm 不等，是正常 NBD2 尾部长度的 23 倍。稳定性分析表明，多管可以承受各种化学和物理条件。这些多管有望在科学的各个领域用作纳米材料。
