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从城市污水中分离出的噬菌体vB-EcoS-95的特性及其极快速的裂解发育揭示

Characterization of Bacteriophage vB-EcoS-95, Isolated From Urban Sewage and Revealing Extremely Rapid Lytic Development.

作者信息

Topka Gracja, Bloch Sylwia, Nejman-Faleńczyk Bożena, Gąsior Tomasz, Jurczak-Kurek Agata, Necel Agnieszka, Dydecka Aleksandra, Richert Malwina, Węgrzyn Grzegorz, Węgrzyn Alicja

机构信息

Department of Molecular Biology University of Gdańsk, Gdańsk, Poland.

Laboratory of Molecular Biology, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Gdańsk, Poland.

出版信息

Front Microbiol. 2019 Jan 15;9:3326. doi: 10.3389/fmicb.2018.03326. eCollection 2018.

DOI:10.3389/fmicb.2018.03326
PMID:30697202
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6340994/
Abstract

Morphological, biological, and genetic characteristics of a virulent phage, named vB-EcoS-95, is reported. This phage was isolated from urban sewage. It was found to infect some strains giving clear plaques. The genome of this phage is composed of 50,910 bp and contains 89 ORFs. Importantly, none of the predicted ORFs shows any similarity with known pathogenic factors that would prevent its use in medicine. Genome sequence analysis of vB-EcoS-95 revealed 74% similarity to genomic sequence of phage pSf-1. Compared to pSf-1, phage vb-EcoS-95 does not infect strains and has an efficient bacteriolytic activity against some strains. One-step growth analysis revealed that this phage has a very short latent period (4 min), and average burst size of 115 plaque forming units per cell, which points to its high infectivity of host cells and strong lytic activity. The bacteriolytic effect of vB-EcoS-95 was tested also on biofilm-producing strains. These results indicate that vB-EcoS-95 is a newly discovered phage that may be potentially used to control the formation of biofilms.

摘要

报道了一种名为vB-EcoS-95的烈性噬菌体的形态学、生物学和遗传学特征。该噬菌体从城市污水中分离得到。发现它能感染一些菌株并产生清晰的噬菌斑。该噬菌体的基因组由50910个碱基对组成，包含89个开放阅读框。重要的是，预测的开放阅读框中没有一个与已知的致病因子显示出任何相似性，这使得它可用于医学领域。vB-EcoS-95的基因组序列分析显示与噬菌体pSf-1的基因组序列有74%的相似性。与pSf-1相比，噬菌体vb-EcoS-95不感染某些菌株，对一些菌株具有高效的溶菌活性。一步生长分析表明，该噬菌体的潜伏期非常短（4分钟），平均裂解量为每个细胞115个噬菌斑形成单位，这表明它对宿主细胞具有高感染性和强裂解活性。还测试了vB-EcoS-95对产生物膜菌株的溶菌作用。这些结果表明，vB-EcoS-95是一种新发现的噬菌体，可能潜在地用于控制生物膜的形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/1f36c6d95843/fmicb-09-03326-g0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/b87dcfe0f7e8/fmicb-09-03326-g0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/f437a2a5e073/fmicb-09-03326-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/1951bec4a7c8/fmicb-09-03326-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/d9bfd759fb1d/fmicb-09-03326-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/93ef8b573740/fmicb-09-03326-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/81886c7c59bb/fmicb-09-03326-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/a3047affc42d/fmicb-09-03326-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/626d1e39dd57/fmicb-09-03326-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/59b6d4c4a8f1/fmicb-09-03326-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/1f36c6d95843/fmicb-09-03326-g0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/b87dcfe0f7e8/fmicb-09-03326-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/ce3c510b7f11/fmicb-09-03326-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/f437a2a5e073/fmicb-09-03326-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/1951bec4a7c8/fmicb-09-03326-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/d9bfd759fb1d/fmicb-09-03326-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/93ef8b573740/fmicb-09-03326-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/81886c7c59bb/fmicb-09-03326-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/a3047affc42d/fmicb-09-03326-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/626d1e39dd57/fmicb-09-03326-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/59b6d4c4a8f1/fmicb-09-03326-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d97/6340994/1f36c6d95843/fmicb-09-03326-g0011.jpg

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