Kaliniene Laura, Truncaitė Lidija, Šimoliūnas Eugenijus, Zajančkauskaitė Aurelija, Vilkaitytė Monika, Kaupinis Algirdas, Skapas Martynas, Meškys Rolandas
Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio al. 7, 10257, Vilnius, Lithuania.
Proteomics Center, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio al. 7, 10257, Vilnius, Lithuania.
Arch Virol. 2018 Jan;163(1):105-114. doi: 10.1007/s00705-017-3589-5. Epub 2017 Oct 10.
A novel low-temperature Escherichia coli phage vB_EcoS_NBD2 was isolated in Lithuania from agricultural soil. With an optimum temperature for plating around 20 °C, vB_EcoS_NBD2 efficiently produced plaques on Escherichia coli NovaBlue (DE3) at a temperature range of 10-30 °C, yet failed to plate at temperatures above 35 °C. Phage vB_EcoS_NBD2 virions have a siphoviral morphology with an isometric head (65 nm in diameter), and a non-contractile flexible tail (170 nm). The 51,802-bp genome of vB_EcoS_NBD2 has a G + C content of 49.8%, and contains 87 probable protein-encoding genes as well as 1 gene for tRNA. Comparative sequence analysis revealed that 22 vB_EcoS_NBD2 ORFs encode unique proteins that have no reliable identity to database entries. Based on homology to biologically defined proteins and/or proteomics analysis, 36 vB_EcoS_NBD2 ORFs were given a putative functional annotation, including 20 genes coding for morphogenesis-related proteins and 13 associated with DNA metabolism. Phylogenetic analysis revealed that vB_EcoS_NBD2 belongs to the subfamily Tunavirinae, but cannot be assigned to any genus currently recognized by ICTV.
一种新型低温大肠杆菌噬菌体vB_EcoS_NBD2于立陶宛从农业土壤中分离得到。vB_EcoS_NBD2的最佳铺板温度约为20°C，它能在10至30°C的温度范围内在大肠杆菌NovaBlue(DE3)上高效产生噬菌斑，但在35°C以上的温度下无法铺板。噬菌体vB_EcoS_NBD2病毒粒子具有长尾噬菌体形态，头部呈等轴状（直径65纳米），尾部为非收缩性柔性尾（170纳米）。vB_EcoS_NBD2的51,802碱基对基因组的G + C含量为49.8%，包含87个可能的蛋白质编码基因以及1个tRNA基因。比较序列分析表明，vB_EcoS_NBD2的22个开放阅读框（ORF）编码的独特蛋白质与数据库条目没有可靠的同源性。基于与生物学定义的蛋白质的同源性和/或蛋白质组学分析，36个vB_EcoS_NBD2的ORF被赋予了假定的功能注释，其中包括20个编码形态发生相关蛋白质的基因和13个与DNA代谢相关的基因。系统发育分析表明，vB_EcoS_NBD2属于Tunavirinae亚科，但无法归入国际病毒分类委员会目前认可的任何属。
