Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
Anal Chim Acta. 2019 May 30;1057:70-79. doi: 10.1016/j.aca.2019.01.024. Epub 2019 Jan 28.
A simple, rapid and high throughput analytical approach with combination of mechanochemical extraction (MCE) and direct analysis in real time mass spectrometry (DART-MS) analysis was developed for the simultaneous determination of multiple chemical components in cigarette fillers. Different kinds of substances including nicotine, cigarette alkaloids, carbohydrates, organic acids, humectants and other additives were successfully extracted using MCE and detected by high resolution DART-MS. Six solvents of various polarities were compared during MCE process and significant differences were observed. Different brands of cigarettes as well as standard research cigarette exhibited distinctive chemical features and DART-MS fingerprints. Principle component analysis showed clear differentiation among different cigarettes extracted with the same solvent and different solvent extracts of the same type of cigarette. The putative chemical formulas were proposed based on accurate m/z values with <10 ppm mass errors. The relative contents of nicotine and other identified substances were compared and significant differences were observed among cigarettes of different locations. The whole procedure of MCE combined with DART-MS only takes minutes from raw cigarette fillers to obtaining the semi-quantitative results. The operation is simple and high throughput, providing an efficient method to analyze cigarette composition, and to establish a methodology to acquire the rapid cigarette fingerprints for quality control.
建立了一种简单、快速、高通量的分析方法，结合机械化学提取（MCE）和实时直接分析质谱（DART-MS）分析，用于同时测定烟用滤嘴中的多种化学成分。采用 MCE 成功提取了尼古丁、卷烟生物碱、碳水化合物、有机酸、保湿剂和其他添加剂等多种物质，并采用高分辨 DART-MS 进行检测。在 MCE 过程中比较了 6 种不同极性的溶剂，观察到了显著差异。不同品牌的香烟以及标准研究香烟表现出独特的化学特征和 DART-MS 指纹。主成分分析表明，用相同溶剂提取的不同香烟以及同一类型香烟用不同溶剂提取的样品之间有明显的区别。根据准确的 m/z 值和 <10 ppm 的质量误差提出了假定的化学公式。比较了不同位置香烟中尼古丁和其他鉴定物质的相对含量，观察到了显著差异。从原始香烟滤嘴到获得半定量结果，MCE 与 DART-MS 相结合的整个过程只需几分钟。操作简单，高通量，为分析香烟成分提供了一种有效的方法，并为建立快速香烟指纹图谱的质量控制方法提供了依据。
