Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
Talanta. 2020 Sep 1;217:121060. doi: 10.1016/j.talanta.2020.121060. Epub 2020 Apr 23.
Particulate matter from mainstream smoke (MSS) is significantly hazardous when inhaled into the human body. An ambient ionization mass spectrometric method, direct analysis in real time mass spectrometry (DART-MS), was applied to rapidly and simultaneously measure multiple particulate components in MSS. A variety of compounds were obtained in seconds, where different types of cigarettes and different solvent extracts generated distinct chemical constituents as validated by principle component analysis. Chemical formula assignment and compound identification were based on accurate m/z values with mass errors <10 ppm. Quantitation of nicotine was achieved using an isotope internal standard with DART-MS. Method validation with chromatographic-MS analysis further proved the advantages of DART-MS with respect to analysis speed and operational simplicity for the direct evaluation of complex samples. DART-MS is feasible for the rapid acquisition of cigarette fingerprints for quality control as well as for quantitative assessment of carcinogens for harm reduction.
主流烟雾（MSS）中的颗粒物在吸入人体时具有显著的危害性。本研究采用环境离子化质谱分析方法，即实时直接分析质谱（DART-MS），对 MSS 中的多种颗粒物成分进行快速同步测量。在数秒内获得了多种化合物，通过主成分分析验证了不同类型的香烟和不同溶剂提取物产生了不同的化学成分。基于准确的 m/z 值（质量误差 <10 ppm）进行化学式赋值和化合物鉴定。采用 DART-MS 中的同位素内标法对尼古丁进行定量。通过与色谱-MS 分析的方法验证，进一步证明了 DART-MS 在直接分析复杂样品时具有速度快、操作简单的优势。DART-MS 可用于快速获取香烟指纹图谱以进行质量控制，以及对减少危害的致癌物进行定量评估。
