The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark.
Cellular Stress Signaling Group, Center for Healthy Aging, University of Copenhagen, Denmark.
Mol Cell Proteomics. 2019 May;18(5):1027-1035. doi: 10.1074/mcp.TIR118.001270. Epub 2019 Mar 4.
Universal proteomics sample preparation is challenging because of the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for nonspecific immobilization on microparticles by protein aggregation capture. To demonstrate the general applicability of this mechanism, we analyzed phosphoproteomes, tissue proteomes, and interaction proteomes as well as dilute secretomes. The findings present a practical, sensitive and cost-effective proteomics sample preparation method.
通用蛋白质组学样品制备具有挑战性，因为生物样品的异质性很高。在这里，我们描述了一种新的机制，该机制利用变性蛋白质固有的不稳定性，通过蛋白质聚集捕获将其非特异性固定在微粒上。为了证明这种机制的普遍适用性，我们分析了磷酸化蛋白质组、组织蛋白质组和相互作用蛋白质组以及稀释的分泌蛋白质组。研究结果提出了一种实用、灵敏和具有成本效益的蛋白质组学样品制备方法。
