Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, West Yuanmingyuan Road, Beijing, China.
Insect Mol Biol. 2019 Oct;28(5):628-636. doi: 10.1111/imb.12578. Epub 2019 Mar 25.
Cotton plants produce gossypol as a major secondary metabolite to resist insect herbivores and pathogens. Helicoverpa armigera may employ multigene families of detoxification enzymes to deal with this metabolite. So far, the strength of the transcriptional response to gossypol detoxification in the cotton bollworms remains poorly understood. Here, we investigated the genomewide transcriptional changes that occur in cotton bollworm larvae after one generation feeding on various host plants (cotton, corn, soybean and chili) or an artificial diet. Six genes potentially involved in detoxification of xenobiotics were highly upregulated in bollworms fed on cotton, and the expression of five of these differed significantly in insects that fed on gossypol diet compared with the artificial diet. When these six genes were downregulated using RNA interference, downregulation only of CYP4L11, CYP6AB9 and CCE001b led to reduced growth of bollworm larvae feeding on gossypol diets. These data suggest that the three genes are involved in response of H. armigera to gossypol of cotton. Our results proved that H. armigera may have a broad mechanism for gossypol detoxification.
棉花植物产生棉酚作为一种主要的次生代谢物，以抵抗昆虫食草动物和病原体。棉铃虫可能利用解毒酶的多基因家族来应对这种代谢物。到目前为止，棉铃虫对棉酚解毒的转录反应强度还知之甚少。在这里，我们研究了在一代幼虫以各种宿主植物（棉花、玉米、大豆和辣椒）或人工饲料喂养后，棉铃虫中发生的全基因组转录变化。在以棉花为食的棉铃虫中，有 6 个可能参与外来化合物解毒的基因被高度上调，其中 5 个基因在以棉酚饮食喂养的昆虫中与人工饮食相比差异显著。当使用 RNA 干扰下调这 6 个基因时，只有 CYP4L11、CYP6AB9 和 CCE001b 的下调导致以棉酚饮食喂养的棉铃虫幼虫生长减少。这些数据表明，这三个基因参与了 H. armigera 对棉花棉酚的反应。我们的结果证明，H. armigera 可能具有广泛的棉酚解毒机制。
