Hafeez Muhammad, Li Xiaowei, Chen Limin, Ullah Farman, Huang Jun, Zhang Zhijun, Zhang Jinming, Siddiqui Junaid Ali, Zhou Shu-Xing, Ren Xiao-Yun, Imran Muhammad, Assiri Mohammed A, Lou Yonggen, Lu Yaobin
State Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Front Plant Sci. 2023 Jan 25;13:1079442. doi: 10.3389/fpls.2022.1079442. eCollection 2022.
The fall armyworm (FAW) is a destructive and polyphagous pest of many essential food crops including maize and rice. The FAW is hard to manage, control, or eradicate, due to its polyphagous nature and voracity of feeding. Here, we report the characterization and functional analysis of the detoxification gene and how larvae use a detoxification mechanism to adapt host plants. Results demonstrated that expression levels were much higher in midgut tissue and the older larvae. Our current studies revealed the enhanced P450 activity in the midguts of larvae after exposure to rice plants as compared to corn plants and an artificial diet. Furthermore, higher mortality was observed in PBO treated larvae followed by the exposure of rice plants as compared to the corn plant. The dsRNA-fed larvae showed downregulation of CYP302A1 gene in the midgut. At the same time, higher mortality, reduced larval weight and shorter developmental time was observed in the dsRNA-fed larvae followed by the exposure of rice plant as compared to the corn plant and DEPC-water treated plants as a control. These results concluded that the inducible P450 enzyme system and related genes could provide herbivores with an ecological opportunity to adapt to diverse host plants by utilizing secondary compounds present in their host plants.
草地贪夜蛾是包括玉米和水稻在内的许多重要粮食作物的一种具有破坏性且食性广泛的害虫。由于其食性广泛和取食贪婪的特性，草地贪夜蛾很难管理、控制或根除。在此，我们报告了解毒基因的特征和功能分析，以及幼虫如何利用解毒机制来适应寄主植物。结果表明，该基因在中肠组织和较老幼虫中的表达水平要高得多。我们目前的研究表明，与玉米植株和人工饲料相比，草地贪夜蛾幼虫接触水稻植株后，其肠道中的P450活性增强。此外，与接触玉米植株相比，用胡椒基丁醚（PBO）处理后的幼虫再接触水稻植株时，观察到更高的死亡率。用dsRNA喂养的幼虫中肠中的CYP302A1基因表达下调。同时，与玉米植株和作为对照的经焦碳酸二乙酯（DEPC）水处理的植株相比，用dsRNA喂养的幼虫再接触水稻植株时，观察到更高的死亡率、幼虫体重减轻和发育时间缩短。这些结果得出结论，可诱导的P450酶系统和相关基因可以为食草动物提供一个生态机会，使其通过利用寄主植物中存在的次生化合物来适应不同的寄主植物。
