Department of Trauma, Hand, Plastic and Reconstructive Surgery, University of Wuerzburg, Oberduerrbacher Str. 6, 97080, Wuerzburg, Germany.
Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Ave, Kelvin Grove, 4059, Australia.
Adv Healthc Mater. 2019 Apr;8(7):e1801326. doi: 10.1002/adhm.201801326. Epub 2019 Mar 5.
Melt electrowriting (MEW) is an additive manufacturing technology that is recently used to fabricate voluminous scaffolds for biomedical applications. In this study, MEW is adapted for the seeding of multicellular spheroids, which permits the easy handling as a single sheet-like tissue-scaffold construct. Spheroids are made from adipose-derived stromal cells (ASCs). Poly(ε-caprolactone) is processed via MEW into scaffolds with box-structured pores, readily tailorable to spheroid size, using 13-15 µm diameter fibers. Two 7-8 µm diameter "catching fibers" near the bottom of the scaffold are threaded through each pore (360 and 380 µm) to prevent loss of spheroids during seeding. Cell viability remains high during the two week culture period, while the differentiation of ASCs into the adipogenic lineage is induced. Subsequent sectioning and staining of the spheroid-scaffold construct can be readily performed and accumulated lipid droplets are observed, while upregulation of molecular markers associated with successful differentiation is demonstrated. Tailoring MEW scaffolds with pores allows the simultaneous seeding of high numbers of spheroids at a time into a construct that can be handled in culture and may be readily transferred to other sites for use as implants or tissue models.
熔融电写入（MEW）是一种最近用于制造生物医学应用的大量支架的增材制造技术。在这项研究中，MEW 被用于接种细胞球，这使得作为单个片状组织-支架结构的处理变得容易。细胞球由脂肪来源的基质细胞（ASCs）制成。聚（ε-己内酯）通过 MEW 加工成具有盒状孔的支架，使用 13-15 µm 直径的纤维，很容易适应细胞球的大小。在支架的底部附近有两根 7-8 µm 直径的“捕捉纤维”穿过每个孔（360 和 380 µm），以防止在接种过程中细胞球丢失。在两周的培养期间，细胞活力仍然很高，同时诱导 ASCs 向成脂谱系分化。随后可以轻松地对细胞球-支架结构进行切片和染色，并观察到积累的脂滴，同时证明与成功分化相关的分子标记物的上调。通过对具有孔的 MEW 支架进行定制，可以同时将大量细胞球接种到一个可以在培养中处理的结构中，并可以很容易地转移到其他部位作为植入物或组织模型使用。
