a Faculty of Sciences, Department of Biology , Karadeniz Technical University , Trabzon , Turkey.
J Microencapsul. 2019 Jan;36(1):1-9. doi: 10.1080/02652048.2019.1572238. Epub 2019 May 14.
In this study, microencapsulation by spray drying was performed to protect spores and crystals of an indigenous isolate of Se13 from environmental stress. The effects of wall material, inlet temperature, and outlet temperature on microencapsulation of -Se13 were investigated using Taguchi's orthogonal array. The most suitable wall material determined as maltodextrin DE10. The optimum inlet and outlet temperatures of spray drier were determined as 160 °C and 70 °C, respectively. The number of viable spores, mean particle size, wetting time, percentage of suspensibility and moisture content of the product produced under optimum conditions were determined as 8.1 × 10 cfu g, 13.462 µm, 25.22 s, 77.66% and 7.29%, respectively. As a result of efficiency studies on in the laboratory conditions, the LC was determined as 1.6 × 10 cfu mL. Microencapsulated -Se13 based bio-pesticide may be registered for the control of and can be tested against other lepidopterans which share the same environment.
在这项研究中，通过喷雾干燥进行微胶囊化，以保护本土分离株 Se13 的孢子和晶体免受环境压力的影响。使用田口正交数组研究了壁材、入口温度和出口温度对 -Se13 微胶囊化的影响。确定麦芽糊精 DE10 为最合适的壁材。喷雾干燥器的最佳入口和出口温度分别确定为 160°C 和 70°C。在最佳条件下生产的产品的活菌数、平均粒径、润湿时间、悬浮率和水分含量分别为 8.1×10cfu g、13.462µm、25.22s、77.66%和 7.29%。在实验室条件下对 进行效率研究的结果表明，LC 为 1.6×10cfu mL。基于微囊化 -Se13 的生物农药可用于防治 和可针对具有相同环境的其他鳞翅目昆虫进行测试。
