Munshi Renuka, Joshi Samidha, Talele Gitanjali, Shah Rajesh
Department of Clinical Pharmacology, TNMC & BYL Nair Charitable Hospital, Mumbai, Maharashtra, India.
Life Force Foundation Trust, Mumbai, Maharashtra, India.
Homeopathy. 2019 Aug;108(3):183-187. doi: 10.1055/s-0039-1678541. Epub 2019 Mar 5.
The authors had previously conducted an in-vitro study to observe the effect of homeopathic medicines on melanogenesis, demonstrating anti-vitiligo potential by increasing the melanin content in murine B16F10 melanoma cells. A similar experiment was performed using further homeopathic preparations sourced from kojic acid (KA), hydrogen peroxide (HO; HP), 6-biopterin (BP), and [Nle, D-Phe]-α-melanocyte-stimulating hormone (NLE), some of which are known to induce vitiligo or melano-destruction at physiological dose.
The homeopathic preparations of BP, KA, NLE, and HP were used in 30c potency. Alcohol and potentized alcohol were used as vehicle controls. Prior to starting the main experiment, the viability of B16F10 melanoma cells after treatment with study preparations was assayed. Melanin content (at 48 h and 96 h) and tyrosinase activity in melanocytes were determined.
At the end of 48 hours, NLE and HP in 30c potency had a significantly greater melanin content ( = 0.015 and = 0.039, respectively) compared with controls; BP and KA in 30c potency had no significant effects. No significant changes were seen at the end of 96 hours. KA, NLE, HP, and vehicle controls showed an inhibition of tyrosinase activity.
The study demonstrated melanogenic effects of two homeopathic preparations. Further research to evaluate the therapeutic efficacy of these medicines is warranted.
作者之前进行了一项体外研究，以观察顺势疗法药物对黑色素生成的影响，通过增加小鼠B16F10黑色素瘤细胞中的黑色素含量来证明其抗白癜风潜力。使用从曲酸（KA）、过氧化氢（HO；HP）、6-生物蝶呤（BP）和[Nle，D-Phe]-α-黑素细胞刺激素（NLE）中提取的其他顺势疗法制剂进行了类似实验，其中一些在生理剂量下已知会诱发白癜风或黑色素破坏。
BP、KA、NLE和HP的顺势疗法制剂采用30c药力。酒精和增效酒精用作赋形剂对照。在开始主要实验之前，测定了用研究制剂处理后B16F10黑色素瘤细胞的活力。测定了黑色素细胞中黑色素含量（48小时和96小时时）和酪氨酸酶活性。
在48小时结束时，与对照组相比，30c药力的NLE和HP的黑色素含量显著更高（分别为=0.015和=0.039）；30c药力的BP和KA没有显著影响。在96小时结束时未观察到显著变化。KA、NLE、HP和赋形剂对照均显示酪氨酸酶活性受到抑制。
该研究证明了两种顺势疗法制剂的黑色素生成作用。有必要进一步研究以评估这些药物的治疗效果。
