Department of Pharmacology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
J Ethnopharmacol. 2012 Jun 14;141(3):1005-11. doi: 10.1016/j.jep.2012.03.047. Epub 2012 Apr 5.
Pyrostegia venusta is a native Brazilian plant which has a variety of uses in traditional folk medicine including the treatment of vitiligo. However, its effectiveness on melanogenesis is not yet elucidated.
This study aimed to investigate the melanogenic activity of hydroalcoholic extracts from the leaves and flowers of P. venusta on murine B16F10 melanoma cells.
Different concentrations of the hydroalcoholic extracts of flowers and leaves of P. venusta were evaluated in trials of spontaneous melanin content (4 days), and cell viability by the MTT assay in murine B16F10 cells, and in the mushroom tyrosinase activity in vitro.
Both extracts, leaves (0.1; 0.3; 1 and 3 μg/mL) and flowers (0.03 and 0.1 μg/mL) increased the melanin content in a concentration dependent manner after 4 days of incubation on melanoma cells. Leaves extract promoted enhancement of melanogenesis with maximum effect of 33.3±3% (3 μg/mL), and the flower extract increased in 23.4±3% (0.1 μg/mL). The cell viability test using MTT showed that in the same tested concentrations of both extracts no cell death was detected. Actually, either extract was not able to cause any change in the tyrosinase activity. HPLC analysis of P. venusta extracts found 0.09% and 1.08% of allantoin on leaves and flowers extracts, respectively.
The leaves and flowers extracts of P. venusta stimulates B16F10 melanogenesis at very low concentrations. These findings support the folk medicinal use of P. venusta on the treatment of hypopigmentation diseases, such as vitiligo.
Pyrostegia venusta 是一种原产于巴西的植物，在传统民间医学中有多种用途，包括治疗白癜风。然而，其对黑色素生成的作用尚未阐明。
本研究旨在探讨 Pyrostegia venusta 的叶和花的水醇提取物对小鼠 B16F10 黑素瘤细胞的黑色素生成活性。
在对小鼠 B16F10 细胞进行自发黑色素含量试验（4 天）和 MTT 试验评估细胞活力以及在体外蘑菇酪氨酸酶活性试验中，评估了 Pyrostegia venusta 的叶和花的水醇提取物的不同浓度。
两种提取物（叶：0.1；0.3；1 和 3μg/mL；花：0.03 和 0.1μg/mL）在孵育 4 天后，均以浓度依赖的方式增加黑色素含量。叶提取物促进黑色素生成增强，最大效果为 33.3±3%（3μg/mL），花提取物增加 23.4±3%（0.1μg/mL）。MTT 细胞活力试验表明，在相同测试浓度下，两种提取物均未检测到细胞死亡。实际上，两种提取物均不能引起酪氨酸酶活性的任何变化。HPLC 分析 Pyrostegia venusta 提取物发现，叶和花提取物中的尿囊素含量分别为 0.09%和 1.08%。
Pyrostegia venusta 的叶和花提取物在非常低的浓度下刺激 B16F10 黑色素生成。这些发现支持 Pyrostegia venusta 在治疗白癜风等色素减退性疾病中的民间药用用途。
