Herbal Medicine Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 34054, Korea.
Research Infrastructure Team, Herbal Medicine Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea.
Molecules. 2020 Jun 3;25(11):2605. doi: 10.3390/molecules25112605.
The accumulation and formation of advanced glycation end products (AGEs) are related to diabetes and age-related disease. C. K. Schneid. (Rosaceae, OSSC) is used traditionally for the treatment of various diseases in Asia. Previous studies have shown that OSSC elicits preventive effects in an in vivo model of diabetes. This study was to evaluate the antiapoptotic effects of dried leaves and twigs of OSSC extract and its major compounds in ARPE-19 cells-spontaneously arising human retinal pigment epithelial cells-under diabetic conditions. To examine the effects of an OSSC extract and its active compounds (acetylvitexin, hyperoside and quercitrin) on apoptosis in methylglyoxal (MG, the active precursor in the formation of AGEs)-treated ARPE-19 cells and the mechanism by which these effects occur, apoptosis was measured using flow cytometry analysis. Protein expression levels of phospho-p53 (p-p53), Bax and Bcl-2 were determined by western blot analyses. The OSSC extract inhibited apoptosis in MG-treated ARPE-19 cells in a dose-dependent manner. The major compounds also reduced the rate of apoptosis. Both the extract and major compounds also inhibited the expression of p-p53 and Bax and increased the levels of Bcl-2 that had been previously reduced by MG treatment. The OSSC extract (0.1 μg/mL) and its major compounds (0.01 μM) attenuated apoptosis in ARPE-19 cells under toxic diabetic conditions by downregulating of expression of p-p53 and Bax. OSSC may serve as an alternative therapy to retard the development of diabetic retinopathy.
晚期糖基化终产物（AGEs）的积累和形成与糖尿病和与年龄相关的疾病有关。 C. K. Schneid.（蔷薇科，OSS）在亚洲传统上被用于治疗各种疾病。先前的研究表明，OSS 在糖尿病的体内模型中具有预防作用。本研究旨在评估 OSS 提取物及其主要化合物在糖尿病条件下对 ARPE-19 细胞（自发产生的人视网膜色素上皮细胞）的抗凋亡作用。为了研究 OSS 提取物及其活性化合物（乙酰基牡荆素、金丝桃苷和槲皮素）对甲基乙二醛（MG，AGE 形成的活性前体）处理的 ARPE-19 细胞凋亡的影响及其作用机制，通过流式细胞术分析测量细胞凋亡。通过 Western blot 分析测定磷酸化 p53（p-p53）、Bax 和 Bcl-2 的蛋白表达水平。OSS 提取物以剂量依赖性方式抑制 MG 处理的 ARPE-19 细胞中的细胞凋亡。主要化合物也降低了细胞凋亡率。提取物和主要化合物还抑制了 p-p53 和 Bax 的表达，并增加了 MG 处理先前降低的 Bcl-2 水平。OSS 提取物（0.1 μg/mL）及其主要化合物（0.01 μM）通过下调 p-p53 和 Bax 的表达，减轻了有毒糖尿病条件下 ARPE-19 细胞的凋亡。OSS 可能作为一种替代疗法，延缓糖尿病性视网膜病变的发展。
