Cosgun A, Bolgul B, Duran N
Department of Paediatric Dentistry, Faculty of Dentistry, University of Hatay Mustafa Kemal, Hatay, Turkey.
Department of Microbiology, Faculty of Dentistry, University of Hatay Mustafa Kemal, Hatay, Turkey.
Niger J Clin Pract. 2019 Mar;22(3):422-431. doi: 10.4103/njcp.njcp_429_18.
The aim of this in vitro study was to investigate the antimicrobial effects, nanohardness, and cytotoxicity of different glass-ionomer restorative materials in dentistry.
The following glass-ionomer restorative materials were used in our study: Argion (VOCO, Germany), Zirconomer (Shofu Inc., Japan), EQUIA Forte (GC, Japan), Fuji II LC capsule (GC, Japan), and Fuji IX GP capsule (GC, Japan). For the cytotoxicity test, a cell culture without release liquids was used as the control group. Microdilution and a disk diffusion test were used to measure the antimicrobial activity. The MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide) assay was used to evaluate cytotoxicity. Nanohardness was measured using a nanoindenter device.
Our study determined that all restorative materials used in this study inhibited bacterial growth in cultures containing 1 × 10 cfu/ml of the bacterial concentrations (Lactobacillus casei and Streptococcus mutans) and that all restorative materials inhibited fungal growth in the cultures containing <1 × 10 cfu/ml of the fungal strains (Candida albicans). IC values obtained for the cytotoxicity test were analyzed using the Chi-square test. After a 72-h incubation period, Zirconomer, EQUIA Forte, Fuji IX GP capsule, and Fuji II LG capsule showed statistically significant lower cell viability values. (P < 0.05). The Kruskal-Wallis analysis was performed on the values obtained from the nanohardness test; the differences between the groups were found to be significant (P < 0.05).
All materials tested showed low antimicrobial activity, and the antifungal activity of these materials was found to be lower than their antimicrobial activity. Only Argion showed non-cytotoxic effect. Beginning with the group with the highest nanohardness values and ending with the lowest, the groups are ranked in the following order: Fuji II LC capsule, EQUIA Forte, Argion, Fuji IX GP capsule, and Zirconomer.
本体外研究旨在调查牙科中不同玻璃离子修复材料的抗菌效果、纳米硬度和细胞毒性。
本研究使用了以下玻璃离子修复材料：Argion（德国VOCO公司）、Zirconomer（日本松风株式会社）、EQUIA Forte（日本GC公司）、Fuji II LC胶囊（日本GC公司）和Fuji IX GP胶囊（日本GC公司）。细胞毒性试验中，将未添加释放液的细胞培养物用作对照组。采用微量稀释法和纸片扩散法测量抗菌活性。采用MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐）法评估细胞毒性。使用纳米压痕装置测量纳米硬度。
我们的研究确定，本研究中使用的所有修复材料均能抑制含有1×10 cfu/ml细菌浓度（干酪乳杆菌和变形链球菌）的培养物中的细菌生长，并且所有修复材料均能抑制含有<1×10 cfu/ml真菌菌株（白色念珠菌）的培养物中的真菌生长。细胞毒性试验获得的IC值采用卡方检验进行分析。孵育72小时后，Zirconomer、EQUIA Forte、Fuji IX GP胶囊和Fuji II LG胶囊的细胞活力值在统计学上显著较低（P<0.05）。对纳米硬度测试获得的值进行Kruskal-Wallis分析；发现各组之间的差异具有统计学意义（P<0.05）。
所有测试材料均显示出较低的抗菌活性，且发现这些材料的抗真菌活性低于其抗菌活性。只有Argion显示出无细胞毒性作用。从纳米硬度值最高的组开始到最低的组结束，各组按以下顺序排列：Fuji II LC胶囊、EQUIA Forte、Argion、Fuji IX GP胶囊和Zirconomer。
