Postgraduate Program in Biological Sciences: Pharmacology and Therapeutics, Universidade Federal Do Rio Grande Do Sul (UFRGS), Porto Alegre, RS, 90050-170, Brazil.
Laboratory of Biomedical Science, Universidade Luterana Do Brasil (ULBRA), Br 285, km 335, Carazinho, RS, 99500-000, Brazil.
Braz J Microbiol. 2021 Sep;52(3):1347-1352. doi: 10.1007/s42770-021-00511-5. Epub 2021 May 5.
We present the structural modification of a commercially available glass ionomer cement by inserting the imidazolium salt 1-n-hexadecyl-3-methylimidazolium chloride (CMImCl), composing a new biomaterial with antifungal biofilm activity. Test specimens were prepared using a commercial glass ionomer cement to which 10 ppm of cetylpyridinium chloride (reference ionic antifungal agent) or CMImCl were added. The feasibility and hypoallergenicity of the new biomaterial were assessed by microhardness plastic deformation and chorioallantoic membrane assays. Colony counting and scanning electron microscopy were used to evaluate the modified specimens' antibiofilm activity against three multidrug-resistant Candida species. The modified glass ionomer cement presented a strong antibiofilm activity against Candida spp., without losing its original micromechanical and hypoallergenic properties, rendering it a promising candidate for further application in dentistry.
我们通过插入咪唑鎓盐 1-十六烷基-3-甲基咪唑氯化物（CMImCl）对一种市售的玻璃离子水门汀进行了结构修饰，制备了一种具有抗真菌生物膜活性的新型生物材料。使用商业玻璃离子水门汀制备测试样本，向其中添加 10ppm 的十六烷基吡啶氯（参考离子抗真菌剂）或 CMImCl。通过显微硬度塑性变形和绒毛尿囊膜试验评估了新材料的可行性和低过敏性。通过集落计数和扫描电子显微镜评估了改性样品对三种多药耐药念珠菌的抗生物膜活性。改性玻璃离子水门汀对念珠菌属表现出强烈的抗生物膜活性，同时保持了其原始的微观机械和低过敏性特性，使其成为在牙科领域进一步应用的有前途的候选材料。
