Mancini Annamaria, Vitucci Daniela, Randers Morten Bredsgaard, Schmidt Jakob Friis, Hagman Marie, Andersen Thomas Rostgaard, Imperlini Esther, Mandola Annalisa, Orrù Stefania, Krustrup Peter, Buono Pasqualina
Dipartimento di Scienze Motorie e del Benessere, Università degli Studi di Napoli Parthenope, Naples, Italy.
CEINGE-Biotecnologie Avanzate, Naples, Italy.
Front Physiol. 2019 Feb 19;10:132. doi: 10.3389/fphys.2019.00132. eCollection 2019.
Aging is a physiological process characterized by a progressive decline of biological functions and an increase in destructive processes in cells and organs. Physical activity and exercise positively affects the expression of skeletal muscle markers involved in longevity pathways. Recently, a new mechanism, autophagy, was introduced to the adaptations induced by acute and chronic exercise as responsible of positive metabolic modification and health-longevity promotion. However, the molecular mechanisms regulating autophagy in response to physical activity and exercise are sparsely described. We investigated the long-term adaptations resulting from lifelong recreational football training on the expression of skeletal muscle markers involved in autophagy signaling. We demonstrated that lifelong football training increased the expression of messengers: RAD23A, HSPB6, RAB1B, TRAP1, SIRT2, and HSBPB1, involved in the auto-lysosomal and proteasome-mediated protein degradation machinery; of RPL1, RPL4, RPL36, MRLP37, involved in cellular growth and differentiation processes; of the Bcl-2, HSP70, HSP90, PSMD13, and of the ATG5-ATG12 protein complex, involved in proteasome promotion and autophagy processes in muscle samples from lifelong trained subjects compared to age-matched untrained controls. In conclusion, our results indicated that lifelong football training positively influence exercise-induced autophagy processes and protein quality control in skeletal muscle, thus promoting healthy aging.
衰老 是一个生理过程，其特征是生物功能逐渐衰退，细胞和器官中的破坏过程增加。体育活动和锻炼对参与长寿途径的骨骼肌标志物的表达有积极影响。最近，一种新的机制——自噬，被引入到急性和慢性运动诱导的适应性变化中，被认为是积极代谢改变和促进健康长寿的原因。然而，关于体育活动和锻炼如何调节自噬的分子机制，目前描述较少。我们研究了终身休闲足球训练对参与自噬信号传导的骨骼肌标志物表达的长期适应性变化。我们证明，与年龄匹配的未训练对照组相比，终身足球训练增加了终身训练受试者肌肉样本中参与自噬体和蛋白酶体介导的蛋白质降解机制的信使分子RAD23A、HSPB6、RAB1B、TRAP1、SIRT2和HSBPB1的表达；增加了参与细胞生长和分化过程的RPL1、RPL4、RPL36、MRLP37的表达；增加了参与蛋白酶体促进和自噬过程的Bcl-2、HSP70、HSP90、PSMD13以及ATG5-ATG12蛋白复合物的表达。总之，我们的结果表明，终身足球训练对运动诱导的骨骼肌自噬过程和蛋白质质量控制有积极影响，从而促进健康衰老。
