永生化小鼠Bmp2基因敲除牙乳头间充质细胞系的建立。
Establishment of an Immortalized Mouse Bmp2 Knockout Dental Papilla Mesenchymal Cell Line.
作者信息
Xu Wen'an, Chen Shuo
机构信息
Department of Developmental Dentistry, School of Dentistry, The University of Texas Health Science at San Antonio, San Antonio, TX, USA.
出版信息
Methods Mol Biol. 2019;1922:13-19. doi: 10.1007/978-1-4939-9012-2_2.
Bone morphogenetic protein 2 (Bmp2) is essential for dentin formation. Bmp2 cKO mice exhibited similar phenotype to dentinogenesis imperfecta (DGI), showing dental pulp exposure, hypomineralized dentin, and delayed odontoblast differentiation. As it is relatively difficult to obtain primary Bmp2 cKO dental papilla mesenchymal cells and to maintain a long-term culture of these primary cells, availability of immortalized deleted Bmp2 dental papilla mesenchymal cells is critical for studying the underlying mechanism of Bmp2 signal in odontogenesis. Here we describe the generation of an immortalized deleted Bmp2 dental papilla mesenchymal (iBmp2-dp) cell line by introducing Cre fluorescent protein (GFP) into the immortalized mouse floxed Bmp2 dental papilla mesenchymal (iBmp2-dp) cells.
骨形态发生蛋白2（Bmp2）对牙本质形成至关重要。Bmp2条件性敲除（cKO）小鼠表现出与牙本质发育不全（DGI）相似的表型，包括牙髓暴露、牙本质矿化不足以及成牙本质细胞分化延迟。由于获取原代Bmp2 cKO牙乳头间充质细胞并对这些原代细胞进行长期培养相对困难，因此永生化的Bmp2缺失牙乳头间充质细胞对于研究Bmp2信号在牙齿发育中的潜在机制至关重要。在此，我们通过将Cre荧光蛋白（GFP）导入永生化的小鼠floxed Bmp2牙乳头间充质（iBmp2-dp）细胞中，描述了永生化的Bmp2缺失牙乳头间充质（iBmp2-dp）细胞系的产生。
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