Suzuki M, Bartlett J D
Division of Biosciences, Ohio State University, College of Dentistry, Columbus, OH, USA.
Methods Mol Biol. 2019;1922:335-340. doi: 10.1007/978-1-4939-9012-2_30.
Chronic fluoride overexposure can cause dental fluorosis. Dental fluorosis is characterized by porous and soft enamel that is vulnerable to erosion and decay. Animal models often contribute to clinical applications by addressing pathogenic questions of disease. To study dental fluorosis, rodent models have been employed because rodent incisors erupt continuously and every stage of enamel development is present along the length of the rodent incisor. Here we present a protocol to induce dental fluorosis in mouse and rat and describe the procedure for extraction of stage specific enamel organ from rat mandibular incisors.
长期氟摄入过量会导致氟斑牙。氟斑牙的特征是牙釉质多孔且质地柔软，容易受到侵蚀和龋坏。动物模型常常通过解决疾病的致病问题来推动临床应用。为了研究氟斑牙，人们采用了啮齿动物模型，因为啮齿动物的门牙会持续生长，且在其门牙的长度上呈现出牙釉质发育的各个阶段。在此，我们展示一种在小鼠和大鼠中诱导氟斑牙的方案，并描述从大鼠下颌切牙中提取特定阶段牙釉质器官的程序。
