Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, School of Life Sciences, Wuhan, Hubei 430079, People's Republic of China.
ARC Centre of Excellence for Translational Photosynthesis and School of Life and Environmental Sciences, University of Sydney, Sydney, New South Wales 2006, Australia.
Environ Microbiol. 2019 Apr;21(4):1497-1510. doi: 10.1111/1462-2920.14582. Epub 2019 Mar 21.
Discovery of red-shifted chlorophyll d and f in cyanobacteria has opened up new avenues to estimate global carbon fixation driven by far-red light. Shaded habitats in humid subtropical forest ecosystems contain an increased proportion of far-red light components relative to residual white light. After an extensive survey of shaded ecosystems within subtropical forests, wide occurrence of red-shifted chlorophyll-producing cyanobacteria was demonstrated by isolated Chl f-producing and Chl d-containing cyanobacteria. Chl f-producing cyanobacteria were classified into the genera of Aphanocapsa and Chroococcidiopsis and two undescribed genera within Leptolyngbyaceae. Newly isolated Chl d-containing Acaryochloris sp. CCNUM4 showed the closest phylogenetic relationship with Acaryochloris species isolated from marine environments. Acaryochloris sp. CCNUM4 produced Chl d as major photopigment, and Chl f-producing cyanobacteria use Chl a under white light conditions but Chl a + f under far-red light conditions. Their habitats are widely distributed in subtropical forest ecosystems and varied from mosses on limestone to macrophyte and freshwater in the streams and ponds. This study presents a significant advance in the knowledge of distribution and diversity of red-shifted chlorophyll-producing cyanobacteria in terrestrial ecosystems. The results suggest that Chl f-producing and Chl d-containing cyanobacteria might be important primary producers in far-red light dominant niches worldwide.
在蓝藻中发现了红移叶绿素 d 和 f，为估计远红光驱动的全球碳固定开辟了新途径。在潮湿亚热带森林生态系统的荫蔽生境中，远红光成分相对于残余白光的比例增加。在对亚热带森林内荫蔽生态系统进行广泛调查后，通过分离出的产 Chl f 和含 Chl d 的蓝藻，证明了产红移叶绿素的蓝藻广泛存在。产 Chl f 的蓝藻被分类为 Aphanocapsa 和 Chroococcidiopsis 属以及 Leptolyngbyaceae 中的两个未描述属。新分离出的含 Chl d 的 Acaryochloris sp. CCNUM4 与从海洋环境中分离出的 Acaryochloris 物种具有最密切的系统发育关系。Acaryochloris sp. CCNUM4 以 Chl d 为主要光色素，产 Chl f 的蓝藻在白光条件下使用 Chl a，但在远红光条件下使用 Chl a + f。它们的栖息地广泛分布在亚热带森林生态系统中，从石灰岩上的苔藓到溪流和池塘中的大型植物和淡水。本研究在陆地生态系统中红移叶绿素产生蓝藻的分布和多样性方面取得了重大进展。研究结果表明，产 Chl f 和含 Chl d 的蓝藻可能是全球远红光优势生境中重要的初级生产者。
