Department of Chemical and Biomedical Engineering, West Virginia University, WV, USA.
Nanoscale. 2019 Mar 14;11(11):4987-4998. doi: 10.1039/c8nr10090j.
This research proposed to create the next generation of versatile electrochemical-based biosensors capable of monitoring target capture and release as dictated by molecular binding or unbinding. The biosensor integrates cellular machines (i.e., microtubules, structural elements of cells and kinesin molecular motors involved in cellular transport) as functional units; its assembly is based on molecular self-assembly and self-recognition. Our results demonstrate that the designed biosensor was capable of allowing detection of binding and unbinding events based on redox reactions at user-controlled electrode interfaces. The analysis also showed that the sensitivity of the designed biosensor or its ability to record such events could be user-controlled at any given time by adjusting the energy source that "fuels" the system.
本研究旨在开发新一代多功能电化学生物传感器，使其能够根据分子结合或解吸的情况来监测目标的捕获和释放。该生物传感器将细胞机器（即微管、细胞的结构元件和参与细胞运输的驱动蛋白分子马达）整合为功能单元；其组装基于分子自组装和自识别。我们的结果表明，所设计的生物传感器能够基于用户控制的电极界面上的氧化还原反应来检测结合和解离事件。分析还表明，设计的生物传感器的灵敏度或其记录此类事件的能力可以通过调整“为系统提供燃料”的能源在任何给定时间进行用户控制。
