Department of Industrial, Manufacturing, and Systems Engineering, Texas Tech University, Lubbock, TX, 79409, USA.
School of Naval Architecture and Ocean Engineering, Huazhong University of Science and Technology, 430074, Wuhan, China.
J Mater Sci Mater Med. 2019 Mar 6;30(3):36. doi: 10.1007/s10856-019-6239-5.
It has been widely recognized that one of the critical limitations in biofabrication of functional tissues/organs is lack of vascular networks which provide tissues and organs with oxygen and nutrients. Biofabrication of 3D vascular-like constructs is a reasonable first step towards successful printing of functional tissues and organs. In this paper, a dynamic optical projection stereolithography system has been implemented to successfully fabricate 3D Y-shaped tubular constructs with living cells encapsulated. The effects of operating conditions on the cure depth of a single layer have been investigated, such as UV intensity, exposure time, and cell density. A phase diagram has been constructed to identify optimal operating conditions. Cell viability immediately after printing has been measured to be around 75%. Post-printing mechanical properties, swelling properties, and microstructures of the gelatin methacrylate hydrogels have been characterized. The resulting fabrication knowledge helps to effectively and efficiently print tissue-engineered vascular networks with complex geometries.
人们普遍认识到，功能组织/器官的生物制造的一个关键限制是缺乏提供组织和器官氧气和营养的血管网络。3D 血管样结构的生物制造是成功打印功能组织和器官的合理的第一步。在本文中，实现了动态光学投影立体光刻系统，以成功制造封装有活细胞的 3D Y 形管状结构。研究了操作条件对单层固化深度的影响，例如 UV 强度、曝光时间和细胞密度。构建了相图以确定最佳操作条件。打印后立即测量细胞活力约为 75%。对明胶甲基丙烯酸酯水凝胶的后打印机械性能、溶胀性能和微观结构进行了表征。所得到的制造知识有助于有效地打印具有复杂几何形状的组织工程血管网络。
