Beijing Academy of Forestry and Pomology Sciences, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
National R&D Center For Fruit Processing, Beijing, China.
Planta. 2019 Jun;249(6):1799-1810. doi: 10.1007/s00425-019-03110-9. Epub 2019 Mar 6.
157 known and 55 novel miRNAs were found in sweet cherry fruit. MiRNA target genes involved in fruit ripening and the differentially expressed miRNAs under CO treatment were identified. MicroRNAs (miRNAs) are short non-coding RNAs and play important functions in many biological processes, including fruit ripening and senescence. In the current study, the high-throughput sequencing and bioinformatics methods were implemented to decipher the miRNAs landscape in sweet cherry fruit. A total of 157 known miRNAs belonging to 50 families and 55 putative novel miRNAs were found. Target genes of the miRNAs were predicted and genes involved in fruit ripening were found, including F-box proteins and TFs such as SPL, TCP, NAC, MYB, ARF and AP2/ERF. And these target genes were further confirmed by degradome sequencing. A regulatory network model was constructed to uncover the miRNAs and their targets involved in fruit ripening and senescence. Importantly, elevated carbon dioxide can significantly postpone the ripening and senescence of sweet cherry fruit and the differentially expressed miRNAs exposed to CO were identified. These miRNAs included miR482j, miR6275, miR164, miR166, miR171, miR393, miR858, miR3627a, miR6284, miR6289 and miR7122b, and some of their functions were linked to fruit ripening. This study was the first report to profile miRNAs in sweet cherry fruit and it would provide more information for further study of miRNA roles in the ripening processes and their regulation mechanism underlying the effects of high carbon dioxide treatment on fruit ripening.
在甜樱桃果实中发现了 157 个已知和 55 个新的 microRNA。鉴定了miRNA 靶基因参与果实成熟和 CO 处理下差异表达的 miRNAs。MicroRNAs（miRNAs）是短的非编码 RNA，在许多生物过程中发挥着重要作用，包括果实成熟和衰老。在本研究中，采用高通量测序和生物信息学方法来揭示甜樱桃果实中的 miRNAs 图谱。总共发现了 157 个已知的 miRNA，属于 50 个家族和 55 个假定的新 miRNA。预测了 miRNA 的靶基因，并发现了与果实成熟有关的基因，包括 F-box 蛋白和 SPL、TCP、NAC、MYB、ARF 和 AP2/ERF 等 TF。这些靶基因通过降解组测序进一步得到证实。构建了一个调控网络模型，以揭示与果实成熟和衰老相关的 miRNAs 及其靶基因。重要的是，高浓度二氧化碳能显著延缓甜樱桃果实的成熟和衰老，并且鉴定了暴露于 CO 下的差异表达 miRNAs。这些 miRNAs 包括 miR482j、miR6275、miR164、miR166、miR171、miR393、miR858、miR3627a、miR6284、miR6289 和 miR7122b，其中一些功能与果实成熟有关。本研究首次对甜樱桃果实中的 miRNAs 进行了分析，为进一步研究 miRNA 在果实成熟过程中的作用及其在高浓度二氧化碳处理对果实成熟影响的调控机制提供了更多信息。
