Bioengineering Department, Yıldız Technical University, Istanbul, Turkey.
Biotechnol Prog. 2019 May;35(3):e2801. doi: 10.1002/btpr.2801. Epub 2019 Mar 18.
Microalgae harvesting via pH induced flocculation along with utilization of recovered medium after flocculation is one of the most economical methods for separating the microalgal biomass in order to reduce the dewatering cost. In this study, optimization of marine and freshwater microalgae flocculation by pH adjustment was investigated via central composite design methodology. One molar of KOH and NaOH solutions were used to increase the pH level of the microalgal culture. Increasing pH value of the medium provided the highest flocculation efficiency up to 92.63 and 86.18% with pH adjusted to 10.5 with KOH and NaOH solutions for marine microalgae Nannochloropsis oculata and freshwater microalgae Chlorella minutissima, respectively. Also, it was revealed that microalgae cells were still alive after flocculation process and their biochemical composition was not changed, and flocculated medium can be used again for the next microalgal production. According to the results, it can be said that this method is cheap and effective, simple to operate and provides the utilization of flocculated medium again.
通过 pH 值诱导的絮凝来收获微藻，同时利用絮凝后的回收培养基，是分离微藻生物质以降低脱水成本的最经济方法之一。在这项研究中，通过中心复合设计方法研究了海洋和淡水微藻的絮凝优化。使用 1 摩尔的 KOH 和 NaOH 溶液来提高微藻培养液的 pH 值。将培养基的 pH 值升高到 10.5，使用 KOH 和 NaOH 溶液分别可使海洋微藻眼点拟微绿球藻和淡水微藻微小隐藻的絮凝效率达到最高，分别为 92.63%和 86.18%。此外，研究还表明，絮凝后微藻细胞仍然存活，其生化组成没有改变，并且可以再次使用絮凝培养基进行下一轮微藻生产。根据研究结果可以得出，该方法廉价有效、操作简单，并可再次利用絮凝后的培养基。
