Department of Bioengineering, Yildiz Technical University, Istanbul, Turkey.
Department of Food Engineering, Yildiz Technical University, Istanbul, Turkey.
Probiotics Antimicrob Proteins. 2023 Jun;15(3):682-693. doi: 10.1007/s12602-021-09904-2. Epub 2022 Jan 10.
Today, the enhancement of spore yields of Bacillus subtilis has considerable interest and has been widely investigated. In this context, studies have been carried out to improve the spore yield as well as the production amount. In order to perform this, optimization studies are conducted for large-scale production of B. subtilis in bioreactors. The prokaryotic structure, high extracellular production potential, lack of pathogenic activity, well-known fermentation technology and short fermentation time are the prominent advantages for the production of B. subtilis in a bioreactor. The Bacillus species produce a wide variety of antifungal and antimicrobial compounds, making them ideal biological control agents. In this study, first, the growth conditions of the medium were investigated and then optimized using the central composite design approach to achieve the highest productivity for the growth of B. subtilis. In the experiments, the effect of temperature of 25, 30 and 35 °C and pH level of 6.0, 7.0 and 8.0 on spore yield was studied. Moreover, the antifungal activity of the B. subtilis culture was investigated against pathogenic fungi: Colletotrichum gloeosporioides, Botrytis cinerea and Aspergillus brasiliensis.
今天，枯草芽孢杆菌孢子产量的提高引起了相当大的兴趣，并得到了广泛的研究。在这种情况下，已经进行了研究以提高孢子产量和生产数量。为此，在生物反应器中进行了枯草芽孢杆菌大规模生产的优化研究。原核结构、高细胞外生产潜力、缺乏致病活性、成熟的发酵技术和较短的发酵时间是在生物反应器中生产枯草芽孢杆菌的突出优势。芽孢杆菌属产生多种抗真菌和抗菌化合物，使其成为理想的生物防治剂。在这项研究中，首先研究了培养基的生长条件，然后使用中心组合设计方法进行优化，以实现枯草芽孢杆菌生长的最高生产力。在实验中，研究了温度为 25、30 和 35°C 以及 pH 值为 6.0、7.0 和 8.0 对孢子产量的影响。此外，还研究了枯草芽孢杆菌培养物对病原菌真菌：炭疽菌、灰葡萄孢和巴西曲霉的抗真菌活性。
