Queen C, Fauci A S
Eur J Immunol. 1986 May;16(5):547-50. doi: 10.1002/eji.1830160515.
Human B cells were cultured without added lymphokines, with interleukin 2 (IL2) or interferon-gamma (IFN-gamma) alone, or with a combination of IL2 and IFN-gamma. Treatment with IL2 alone induced differentiation of the B cells, as shown by an increase in the number of immunoglobulin (Ig)-containing cells and plaque-forming cells, and by a larger amount of Ig secreted into the medium. In most of the induced Ig-containing cells, heavy chains but not light chains were detectable by cytoplasmic staining. Treatment with IFN-gamma alone did not stimulate B cell differentiation. However, a combination of IFN-gamma and IL2 was more effective than IL2 alone in inducing B cell differentiation, as measured by further increases in the number of plaque-forming cells and in total Ig secretion. Furthermore, after treatment with both IL2 and IFN-gamma, most cells that contained cytoplasmic heavy chains also contained cytoplasmic light chains. We conclude that IFN-gamma acts in synergy with IL2 in B cell differentiation by enhancing light chain synthesis, leading to secretion of Ig molecules.
人B细胞在不添加淋巴因子的情况下培养，或单独添加白细胞介素2（IL2）或干扰素-γ（IFN-γ），或同时添加IL2和IFN-γ。单独用IL2处理可诱导B细胞分化，表现为含免疫球蛋白（Ig）的细胞和噬斑形成细胞数量增加，以及分泌到培养基中的Ig量增多。在大多数诱导产生的含Ig细胞中，通过细胞质染色可检测到重链，但检测不到轻链。单独用IFN-γ处理不会刺激B细胞分化。然而，通过噬斑形成细胞数量和总Ig分泌的进一步增加来衡量，IFN-γ和IL2联合使用比单独使用IL2在诱导B细胞分化方面更有效。此外，在用IL2和IFN-γ处理后，大多数含有细胞质重链的细胞也含有细胞质轻链。我们得出结论，IFN-γ通过增强轻链合成与IL2协同作用于B细胞分化，从而导致Ig分子的分泌。
