O'Flynn K, Knott L J, Russul-Saib M, Abdul-Gaffar R, Morgan G, Beverley P C, Linch D C
Eur J Immunol. 1986 May;16(5):580-4. doi: 10.1002/eji.1830160521.
Antigen-induced stimulation of T cells is mediated via the CD3 antigen receptor (Ti) complex and monoclonal antibodies (mAb) reacting with CD3 and Ti result in rapid intracellular Ca2+ mobilization, followed by monocyte-dependent proliferation. Combinations of mAb to CD2, the sheep red blood cell receptor, also mobilize calcium and induce mitogenesis and purified phytohemagglutinin (PHA) stimulates T cells predominantly by interaction with this molecule. It has been suggested that activation via CD2 requires the presence of CD3 and that the hydrophobic epsilon chain of CD3 is the T cell calcium channel. To investigate this further we have obtained large numbers of natural killer (NK) cells which express CD2 but not CD3 from a patient with a chronic expansion of this lymphocyte subpopulation. It is shown that calcium mobilization can be induced in these cells by mAb to CD2 and purified PHA but not by anti-CD3 mAb. This indicates that calcium mobilization can be induced via the CD2 molecule in NK cells not expressing CD3 and that activation through CD2 is separate from the antigen receptor CD3 pathway.
抗原诱导的T细胞刺激是通过CD3抗原受体（Ti）复合物介导的，与CD3和Ti反应的单克隆抗体（mAb）会导致细胞内Ca2+迅速动员，随后是单核细胞依赖性增殖。与绵羊红细胞受体CD2反应的单克隆抗体组合也能动员钙并诱导有丝分裂，纯化的植物血凝素（PHA）主要通过与该分子相互作用来刺激T细胞。有人提出，通过CD2激活需要CD3的存在，并且CD3的疏水ε链是T细胞钙通道。为了进一步研究这一点，我们从一名该淋巴细胞亚群慢性扩增的患者中获得了大量表达CD2但不表达CD3的自然杀伤（NK）细胞。结果表明，针对CD2的单克隆抗体和纯化的PHA可在这些细胞中诱导钙动员，但抗CD3单克隆抗体则不能。这表明在不表达CD3的NK细胞中，可通过CD2分子诱导钙动员，并且通过CD2的激活与抗原受体CD3途径是分开的。
