通过快速免疫选择程序对T细胞红细胞受体CD2抗原进行分子克隆。
Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.
作者信息
Seed B, Aruffo A
出版信息
Proc Natl Acad Sci U S A. 1987 May;84(10):3365-9. doi: 10.1073/pnas.84.10.3365.
A cDNA encoding the CD2 antigen has been isolated by a highly efficient technique based on transient expression in COS cells and adherence of cells expressing surface antigen to antibody-coated dishes. COS cells expressing a CD2 cDNA isolated by this method readily formed rosettes with sheep as well as human and other mammalian erythrocytes. Pretreatment of transfected COS cells with anti-CD2 antibody, or pretreatment of human erythrocytes with anti-LFA-3 antibody, abolished rosette formation.
通过一种高效技术分离出了编码CD2抗原的互补DNA（cDNA），该技术基于在COS细胞中的瞬时表达以及表达表面抗原的细胞与抗体包被培养皿的黏附。用这种方法分离出的表达CD2 cDNA的COS细胞能轻易地与绵羊以及人类和其他哺乳动物的红细胞形成花结。用抗CD2抗体预处理转染的COS细胞，或者用抗淋巴细胞功能相关抗原3（LFA-3）抗体预处理人类红细胞，可消除花结形成。
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