丹酚酸B通过调节mRNA、环状RNA和长链非编码RNA的表达，在高脂饮食诱导的肥胖小鼠中发挥抗肥胖作用。

Salvianolic acid B plays an anti-obesity role in high fat diet-induced obese mice by regulating the expression of mRNA, circRNA, and lncRNA.

作者信息

An Tian, Zhang Jing, Lv Bohan, Liu Yufei, Huang Jiangpinghao, Lian Juan, Wu Yanxiang, Gao Sihua, Jiang Guangjian

机构信息

Traditional Chinese Medicine School, Beijing University of Chinese Medicine, Beijing, China.

Department of Endocrinology, Tangshan Workers Hospital, Tangshan, China.

出版信息

PeerJ. 2019 Feb 28;7:e6506. doi: 10.7717/peerj.6506. eCollection 2019.

BACKGROUND

Adipose tissue plays a central role in obesity-related metabolic diseases such as type 2 diabetes. Salvianolic acid B (Sal B), a water-soluble ingredient derived from , has been shown to reduce obesity and obesity-related metabolic diseases by suppressing adipogenesis. However, the role of Sal B in white adipose tissue (WAT) is not yet clear.

METHODS

Illumina Hiseq 4000 was used to study the effects of Sal B on the expression of long non-coding RNA (lncRNA) and circular RNA (circRNA) in epididymal white adipose tissue induced by a high fat diet in obese mice.

RESULTS

RNA-Seq data showed that 234 lncRNAs, 19 circRNAs, and 132 mRNAs were differentially expressed in WAT under Sal B treatment. The up-regulated protein-coding genes in WAT of the Sal B-treated group were involved in the insulin resistance pathway, while the down-regulated genes mainly participated in the IL-17 signaling pathway. Other pathways may play an important role in the formation and differentiation of adipose tissue, such as B cell receptor signaling. Analysis of the lncRNA-mRNA network provides potential targets for lncRNAs in energy metabolism. We speculate that Sal B may serve as a potential therapeutic approach for obesity.

背景

脂肪组织在肥胖相关的代谢性疾病如2型糖尿病中起核心作用。丹酚酸B（Sal B）是一种从[来源未给出]中提取的水溶性成分，已被证明可通过抑制脂肪生成来减轻肥胖及肥胖相关的代谢性疾病。然而，Sal B在白色脂肪组织（WAT）中的作用尚不清楚。

方法

使用Illumina Hiseq 4000研究Sal B对肥胖小鼠高脂饮食诱导的附睾白色脂肪组织中长链非编码RNA（lncRNA）和环状RNA（circRNA）表达的影响。

结果

RNA测序数据显示，在Sal B处理下，WAT中有234种lncRNA、19种circRNA和132种mRNA差异表达。Sal B处理组WAT中上调的蛋白质编码基因参与胰岛素抵抗途径，而下调的基因主要参与IL-17信号通路。其他途径可能在脂肪组织的形成和分化中起重要作用，如B细胞受体信号通路。lncRNA-mRNA网络分析为lncRNA在能量代谢中的作用提供了潜在靶点。我们推测Sal B可能是一种潜在的肥胖治疗方法。