Takahashi Y, Miyamoto K, Hasegawa Y, Igarashi M
Nihon Naibunpi Gakkai Zasshi. 1986 Feb 20;62(2):126-39. doi: 10.1507/endocrine1927.62.2_126.
Recently, Miyamoto et al and King et al, independently isolated chicken LH-RH and determined its chemical structure as [Gln8] LH-RH. In this report, the in vivo effect of synthetic chicken LH-RH on the release of LH and FSH and ovulation in rats was investigated. A single i.v. injection of chicken LH-RH, as well as mammalian LH-RH, induced a significant increase of plasma LH levels at 15 min after injection in adult male rats. Plasma LH levels then declined at 30 min to 60 min and returned to basal levels at 120 min. The biological potency of chicken LH-RH estimated from plasma LH levels at 15 min after injection in adult male rats by parallel line assay was 4.1% of that of mammalian LH-RH. It was likely that the activity of chicken LH was shorter in duration on the LH secretion as compared with that of mammalian LH-RH because of quickly decreased plasma LH levels. Plasma FSH levels were also increased, but the increment of FSH was not so obvious as compared with that of LH. Plasma FSH levels reached a plateau at 15 min and did not return to basal levels at 120 min in either the chicken LH-RH injection groups or the mammalian LH-RH injection groups. The chicken LH-RH was able to induce an increase of plasma LH and FSH and ovulation in pentobarbital-blocked, proestrous female rats. The biological potency of chicken LH-RH estimated from plasma LH levels at 15 min after injection in pentobarbital-blocked, proestrous female rats was 2.2% that of mammalian LH-RH. The ovulation-inducing potency of chicken LH-RH estimated from ED50 was about 2.1% of that of mammalian LH-RH. It is noteworthy that chicken LH-RH has a LH and FSH releasing and ovulation inducing-activity on rats in vivo, but its biological potency is weaker and of shorter duration compared with that of mammalian LH-RH, although the chemical structure of chicken LH-RH is different from that of mammalian LH-RH.
最近，宫本等人和金等人分别独立分离出鸡促黄体生成素释放激素（LH-RH），并确定其化学结构为[谷氨酰胺8]LH-RH。在本报告中，研究了合成鸡LH-RH对大鼠促黄体生成素（LH）和促卵泡生成素（FSH）释放及排卵的体内作用。单次静脉注射鸡LH-RH以及哺乳动物LH-RH，均可使成年雄性大鼠注射后15分钟时血浆LH水平显著升高。随后血浆LH水平在30分钟至60分钟时下降，并在120分钟时恢复至基础水平。通过平行线测定法，根据成年雄性大鼠注射后15分钟时的血浆LH水平估算，鸡LH-RH的生物活性为哺乳动物LH-RH的4.1%。由于血浆LH水平迅速下降，与哺乳动物LH-RH相比，鸡LH-RH对LH分泌的活性持续时间可能较短。血浆FSH水平也有所升高，但与LH相比，FSH的升高并不明显。在鸡LH-RH注射组和哺乳动物LH-RH注射组中，血浆FSH水平在15分钟时达到平台期，120分钟时均未恢复至基础水平。鸡LH-RH能够使戊巴比妥麻醉的动情前期雌性大鼠血浆LH和FSH升高并诱导排卵。根据戊巴比妥麻醉的动情前期雌性大鼠注射后15分钟时的血浆LH水平估算，鸡LH-RH的生物活性为哺乳动物LH-RH的2.2%。根据半数有效剂量（ED50）估算，鸡LH-RH的排卵诱导活性约为哺乳动物LH-RH的2.1%。值得注意的是，鸡LH-RH在体内对大鼠具有LH和FSH释放及排卵诱导活性，但其生物活性比哺乳动物LH-RH弱，持续时间也较短，尽管鸡LH-RH的化学结构与哺乳动物LH-RH不同。
